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Microfluidics for Molecular Measurements and Quantitative Distributable Diagnostics


Selck, David Anthony (2017) Microfluidics for Molecular Measurements and Quantitative Distributable Diagnostics. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/Z9ZC80XT.


A major challenge in global health care is a lack of portable and affordable quantitative diagnostic devices. This is because classic quantification of biomolecules is typically performed using kinetic assays that require strict control only found in controlled laboratory environments. By using the power of microfluidics, quantitative assays can be performed robustly in a "digital" format that is decoupled from precise kinetics through highly parallelized qualitative reactions. The benefits of performing quantitative assays in a digital format extend beyond just assay robustness to reduction of instrumental complexity, increase in quantitative precision, and an increase in the amount of information that can be gained from a single experiment. These microfluidic architectures, however, are not limited to usage in scenarios of quantification of biomolecules. These architectures can also potentially be extended to answering complex biological questions in single cells, such as determining the 3-dimensional organization of nuclear DNA and RNA.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Microfluidics, PCR, Digital LAMP, LAMP, Real-time PCR, Single-cell sequencing
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Ismagilov, Rustem
Thesis Committee:
  • Cai, Long (chair)
  • Guttman, Mitchell
  • Clemons, William M.
  • Ismagilov, Rustem F.
Defense Date:8 July 2016
Non-Caltech Author Email:david.selck (AT)
Funding AgencyGrant Number
Defense Advanced Research Projects Agency (DARPA)HR0011-11-2-0006
National Institutes of Health (NIH) NRSA Training Grant5T32GM07616NSF
National Institutes of Health (NIH)U01HL130007
Achievement Rewards for College Scientists (ARCS)UNSPECIFIED
Record Number:CaltechTHESIS:07292016-195927111
Persistent URL:
Related URLs:
URLURL TypeDescription adapted for ch. 2 adapted for ch. 3 adapted for ch. 4 adapted for ch. 6 adapted for ch. 7
Selck, David Anthony0000-0002-0591-4165
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9898
Deposited By: David Selck
Deposited On:08 Jun 2017 23:07
Last Modified:04 Oct 2019 00:14

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