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Microfluidics for Molecular Measurements and Quantitative Distributable Diagnostics

Citation

Selck, David Anthony (2017) Microfluidics for Molecular Measurements and Quantitative Distributable Diagnostics. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/Z9ZC80XT. http://resolver.caltech.edu/CaltechTHESIS:07292016-195927111

Abstract

A major challenge in global health care is a lack of portable and affordable quantitative diagnostic devices. This is because classic quantification of biomolecules is typically performed using kinetic assays that require strict control only found in controlled laboratory environments. By using the power of microfluidics, quantitative assays can be performed robustly in a "digital" format that is decoupled from precise kinetics through highly parallelized qualitative reactions. The benefits of performing quantitative assays in a digital format extend beyond just assay robustness to reduction of instrumental complexity, increase in quantitative precision, and an increase in the amount of information that can be gained from a single experiment. These microfluidic architectures, however, are not limited to usage in scenarios of quantification of biomolecules. These architectures can also potentially be extended to answering complex biological questions in single cells, such as determining the 3-dimensional organization of nuclear DNA and RNA.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Microfluidics, PCR, Digital LAMP, LAMP, Real-time PCR, Single-cell sequencing
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Ismagilov, Rustem
Thesis Committee:
  • Cai, Long (chair)
  • Guttman, Mitchell
  • Clemons, William M.
  • Ismagilov, Rustem F.
Defense Date:8 July 2016
Non-Caltech Author Email:david.selck (AT) gmail.com
Funders:
Funding AgencyGrant Number
Defense Advanced Research Projects Agency (DARPA)HR0011-11-2-0006
National Institutes of Health (NIH) NRSA Training Grant5T32GM07616NSF
National Institutes of Health (NIH)U01HL130007
Achievement Rewards for College Scientists (ARCS)UNSPECIFIED
Record Number:CaltechTHESIS:07292016-195927111
Persistent URL:http://resolver.caltech.edu/CaltechTHESIS:07292016-195927111
DOI:10.7907/Z9ZC80XT
Related URLs:
URLURL TypeDescription
http://dx.dloi.org/0.1021/ac4030413DOIArticle adapted for ch. 2
http://dx.doi.org/10.1021/acsnano.5b07338DOIArticle adapted for ch. 3
http://dx.doi.org/10.1039/C4LC00910JDOIArticle adapted for ch. 4
http://dx.doi.org/10.1002/anie.201403035DOIArticle adapted for ch. 6
http://dx.doi.org/10.1093/nar/gkv877DOIArticle adapted for ch. 7
ORCID:
AuthorORCID
Selck, David Anthony0000-0002-0591-4165
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9898
Collection:CaltechTHESIS
Deposited By: David Selck
Deposited On:08 Jun 2017 23:07
Last Modified:08 Jun 2018 18:19

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