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Applications of Computational Protein Design to Red Fluorescent Proteins

Citation

Moore, Matthew Michaels (2013) Applications of Computational Protein Design to Red Fluorescent Proteins. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/6876-A570. http://resolver.caltech.edu/CaltechTHESIS:09132012-234333515

Abstract

The research comprising this thesis is presented in three chapters divided into two parts. Part One, Chapters 2 and 3, of the thesis focuses on the application of rational design and CPD to the core residues of FPs. Chapter 2 applies CPD to the well known red fluorescent protein mCherry. Design hypotheses in this work were driven by a desire to red-shift the fluorescence emission of the parent protein. Chapter 3 takes the most successful results from the mCherry system and attempts to applies these results to the far-red FP mPlum. These two proteins, mCherry and mPlum, share a directed evolution parent, mRFP1. Part Two, consisting of Chapter 4, presents the beginnings of a comprehensive study into the applications CPD for designing FP surfaces. The system used is based on DsRed, the oligomeric parent of monomer mCherry. Professional and personal acknowledgments conclude the thesis.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:mCherry, mPlum, Fluorescent Protein, Computational Protein Design
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Biochemistry and Molecular Biophysics
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Mayo, Stephen L.
Thesis Committee:
  • Hsieh-Wilson, Linda C. (chair)
  • Rees, Douglas C.
  • Arnold, Frances Hamilton
  • Mayo, Stephen L.
Defense Date:13 September 2012
Funders:
Funding AgencyGrant Number
DARPA Protein Design ProcessesUNSPECIFIED
Fonds québécois de la recherche sur la nature et les technologiesUNSPECIFIED
NIHUNSPECIFIED
National Institute of General Medical SciencesP41GM103393
National Center for Research ResourcesP41RR001209
Gordon and Betty Moore FoundationUNSPECIFIED
Record Number:CaltechTHESIS:09132012-234333515
Persistent URL:http://resolver.caltech.edu/CaltechTHESIS:09132012-234333515
DOI:10.7907/6876-A570
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.1013910107 DOIUNSPECIFIED
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7204
Collection:CaltechTHESIS
Deposited By: Matthew Moore
Deposited On:11 Jan 2013 21:08
Last Modified:26 Apr 2019 18:01

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