I. Studies on Mixed Anhydride Systems. II. Studies of the Alpha-Chymotrypsin Catalyzed Hydrolysis of Specific Substrates

Citation

Mower, Howard Frederick (1956) I. Studies on Mixed Anhydride Systems. II. Studies of the Alpha-Chymotrypsin Catalyzed Hydrolysis of Specific Substrates. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/031V-1769. https://resolver.caltech.edu/CaltechETD:etd-06162004-132607

Abstract

The synthesis and purification of p-nitrobenzoic-acetic mixed anhydride, p-methoxybenzoic-acetic mixed anhydride and benzoic-acetic mixed anhydride is described. The thermodynamic constants for the disproportionation reaction have been determined. A mechanism for this reaction is discussed. Measurements of the interaction of acetic and benzoic acids with the acetic-benzoic anhydride system have been made. The cleavage of the mixed anhydrides with nucleophilic agents is described. The synthesis of the following compounds is described: N-trimethylacetyl-L-tyrosinhydrazide, N-dichloroacetyl-L-tyrosinhydrazide, N-formyl-L-tyrosinhydrazide, N-trichloroacetyl-L-tyrosinamide and N-acetyl-L-cysteic acid carboxylamide. The kinetic constants for the [alpha] chymotrypsin hydrolysis of these substances are reported.

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