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The Changing Mouse Embryo Transcriptome at Whole Tissue and Single-Cell Resolution

Citation

He, Peng (2019) The Changing Mouse Embryo Transcriptome at Whole Tissue and Single-Cell Resolution. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/35S4-HG18. https://resolver.caltech.edu/CaltechTHESIS:02192019-004236200

Abstract

Mammalian histogenesis is a sophisticated process of coordinated changes of cellular composition governed by selective gene expression. This thesis focuses on the systematic application of modern RNA-seq methods to histogenesis processes in developing mouse embryos. Most of the work presented here is conducted as part of the ENCODE (ENCyclopedia Of DNA Elements) Project. Chapter 1 introduces the current advances of transcriptome studies on tissue development. Chapter 2 discusses a large-scale study on the whole-tissue transcriptome of 12 embryonic tissues at up to 8 time points and 5 additional perinatal tissues. Coherent themes of biological function and underlying regulatory mechanisms are revealed from the large-scale analysis. Chapter 3 presents a high-resolution single-cell RNAseq study focused on the developing forelimb of the mouse embryo. This approach enables the assignment of differential genes to corresponding lineages and provides an even more accurate picture of RNA level patterns and regulatory modes. Finally, whole-tissue and single-cell methods are compared, contrasted, and integrated in Chapter 4 to extrapolate from the main discoveries of this thesis.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Transcriptomics; RNA; Development; Gene regulation; Molecular; Biology; Computational Biology; Genomics
Degree Grantor:California Institute of Technology
Division:Biology and Biological Engineering
Major Option:Biology
Thesis Availability:Restricted to Caltech community only
Research Advisor(s):
  • Wold, Barbara J.
Thesis Committee:
  • Pachter, Lior S. (chair)
  • Sternberg, Paul W.
  • Fejes Toth, Katalin
  • Thomson, Matthew
  • Guttman, Mitchell
  • Wold, Barbara J.
Defense Date:29 October 2018
Record Number:CaltechTHESIS:02192019-004236200
Persistent URL:https://resolver.caltech.edu/CaltechTHESIS:02192019-004236200
DOI:10.7907/35S4-HG18
ORCID:
AuthorORCID
He, Peng0000-0002-2457-3554
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11396
Collection:CaltechTHESIS
Deposited By: Peng He
Deposited On:22 Feb 2019 16:53
Last Modified:26 Oct 2020 16:48

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