CaltechTHESIS
  A Caltech Library Service

Biochemical and biophysical characterizations of immunoglobulin superfamily receptors neogenin and L1

Citation

Yang, Fan (2011) Biochemical and biophysical characterizations of immunoglobulin superfamily receptors neogenin and L1. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechTHESIS:10242010-143824484

Abstract

Immunoglobulin (Ig) superfamily receptors function in a wide variety of developmental and metabolic processes. We are particularly interested to characterize two Ig superfamily receptors neogenin and L1. The first chapter of the thesis gives a brief review of the biological significance of neogenin and L1 and what has been learned in their functions. In Chapter 2, we described the localization of the hemojuvelin-binding epitope of neogenin to the membrane proximal fifth and sixth fibronectin type III (FNIII) domains, with the sixth FNIII domain contributing the majority of the binding. Chapter 3 presents the crystal structure of this hemojuvelin-binding fragment at 1.8 Å, revealing a nearly linear domain arrangement. Hemojuvelin binding sites have been mapped to one face of the sixth FNIII domain based on sequence alignment between neogenin and DCC (Deleted in Colorectal Cancer), a molecule related to neogenin but does not bind to hemojuvelin. These results should also be informative in understanding the interaction between neogenin and repulsive guidance molecule (RGM), the closest homologue of hemojuvelin. The interaction between neogenin and RGM is known to regulate neuronal survival. Chapter 4, the second part of the thesis, describes our studies of L1-mediated homophilic adhesion using biophysical approaches. We built a basis shape model to describe L1-mediated homophilic adhesion between L1-coated giant unilamellar vesicles and flat substrate. Using confocal microscopy techniques, we were able to reconstruct the three-dimensional shape of an adhered vesicle. We developed an algorithm in order to derive adhesion strength from the configurations of adhered vesicles based on our basis shape model using energy minimization approach.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:immunoglobulin superfamily; hemojuvelin; neogenin; iron homeostasis; L1; cell adhesion; membrane mechanics
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Bjorkman, Pamela J.
Thesis Committee:
  • Rees, Douglas C. (chair)
  • Phillips, Robert B.
  • Kuppermann, Aron
  • Bjorkman, Pamela J.
Defense Date:10 September 2010
Record Number:CaltechTHESIS:10242010-143824484
Persistent URL:http://resolver.caltech.edu/CaltechTHESIS:10242010-143824484
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6156
Collection:CaltechTHESIS
Deposited By: Fan Yang
Deposited On:31 May 2011 19:22
Last Modified:16 Apr 2013 23:39

Thesis Files

[img]
Preview
PDF (Full thesis) - Final Version
See Usage Policy.

94Mb
[img]
Preview
PDF (Title)
See Usage Policy.

166Kb
[img]
Preview
PDF (Chapter 1: Introduction) - Final Version
See Usage Policy.

2351Kb
[img]
Preview
PDF (Chapter 2) - Final Version
See Usage Policy.

21Mb
[img]
Preview
PDF (Chapter 3) - Final Version
See Usage Policy.

10Mb
[img]
Preview
PDF (Chapter 4) - Final Version
See Usage Policy.

8Mb
[img]
Preview
PDF (Appendix A) - Final Version
See Usage Policy.

19Mb
[img]
Preview
PDF (Appendix B) - Final Version
See Usage Policy.

31Mb
[img]
Preview
PDF (Appendix C) - Final Version
See Usage Policy.

118Kb

Repository Staff Only: item control page