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Development of Microfluidic Devices with the Use of Nanotechnology to Aid in the Analysis of Biological Systems Including Membrane Protein Separation, Single Cell Analysis, and Genetic Markers

Citation

Goldberg, Mark David (2015) Development of Microfluidic Devices with the Use of Nanotechnology to Aid in the Analysis of Biological Systems Including Membrane Protein Separation, Single Cell Analysis, and Genetic Markers. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/Z9P848V6. http://resolver.caltech.edu/CaltechTHESIS:06022015-150554177

Abstract

Computation technology has dramatically changed the world around us; you can hardly find an area where cell phones have not saturated the market, yet there is a significant lack of breakthroughs in the development to integrate the computer with biological environments. This is largely the result of the incompatibility of the materials used in both environments; biological environments and experiments tend to need aqueous environments. To help aid in these development chemists, engineers, physicists and biologists have begun to develop microfluidics to help bridge this divide. Unfortunately, the microfluidic devices required large external support equipment to run the device. This thesis presents a series of several microfluidic methods that can help integrate engineering and biology by exploiting nanotechnology to help push the field of microfluidics back to its intended purpose, small integrated biological and electrical devices. I demonstrate this goal by developing different methods and devices to (1) separate membrane bound proteins with the use of microfluidics, (2) use optical technology to make fiber optic cables into protein sensors, (3) generate new fluidic devices using semiconductor material to manipulate single cells, and (4) develop a new genetic microfluidic based diagnostic assay that works with current PCR methodology to provide faster and cheaper results. All of these methods and systems can be used as components to build a self-contained biomedical device.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Biology Nanotechnology
Degree Grantor:California Institute of Technology
Division:Biology and Biological Engineering
Major Option:Biology
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Scherer, Axel
Group:Kavli Nanoscience Institute
Thesis Committee:
  • Davidson, Eric H. (chair)
  • Campbell, Judith L.
  • Elowitz, Michael B.
  • Kartalov, Emil P.
  • Scherer, Axel
Defense Date:27 May 2015
Non-Caltech Author Email:mgold20000 (AT) gmail.com
Record Number:CaltechTHESIS:06022015-150554177
Persistent URL:http://resolver.caltech.edu/CaltechTHESIS:06022015-150554177
DOI:10.7907/Z9P848V6
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8971
Collection:CaltechTHESIS
Deposited By: Mark Goldberg
Deposited On:04 Jun 2015 22:57
Last Modified:27 Jul 2018 02:17

Thesis Files

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