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Microfabricated Tools and Engineering Methods for Sensing Bioanalytes


Rajagopal, Aditya (2014) Microfabricated Tools and Engineering Methods for Sensing Bioanalytes. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/Z9W9575D.


There is a convergence between the needs of the medical community and the capabilities of the engineering community. For example, the scale of biomedical devices and sensors allow for finer, more cost-effective quantification of biological and chemical targets. By using micro-fabrication techniques, we design and demonstrate a variety of microfluidic sensors and actuators that allow us to interact with a biochemical environment. We demonstrate the performance of microfluidic blood-filtrations chips, immune-diagnostic assays, and evaporative coolers. Furthermore, we show how micro-fabricated platinum filaments can be used for highly localized heating and temperature measurement. We demonstrate that these filaments can be used as miniature IR spectroscopic sources. Finally, we describe and demonstrate novel combinatorial coding methods for increasing the information extracted from biochemical reactions. We show proof-principle of these techniques in the context of Taqman PCR as well as persistence length PCR.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:nanofabrication, PCR, combinatorial coding
Degree Grantor:California Institute of Technology
Division:Engineering and Applied Science
Major Option:Electrical Engineering
Awards:Demetriades-Tsafka-Kokkalis Prize in Entrepreneurship or Related Fields, 2013
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Scherer, Axel
Thesis Committee:
  • Tombrello, Thomas A. (chair)
  • Fraser, Scott E.
  • Vaidyanathan, P. P.
  • Rutledge, David B.
  • Baltimore, David L.
Defense Date:22 July 2013
Record Number:CaltechTHESIS:06052014-214022941
Persistent URL:
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8491
Deposited By: Aditya Rajagopal
Deposited On:10 Mar 2017 17:30
Last Modified:27 May 2021 00:16

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