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A cocktail of thermally stable, chemically synthesized capture agents for the efficient detection of anti-gp41 antibodies from human sera and techniques

Citation

Pfeilsticker, Jessica A. (2014) A cocktail of thermally stable, chemically synthesized capture agents for the efficient detection of anti-gp41 antibodies from human sera and techniques. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechTHESIS:05262014-170142267

Abstract

This thesis reports on a method to improve in vitro diagnostic assays that detect immune response, with specific application to HIV-1. The inherent polyclonal diversity of the humoral immune response was addressed by using sequential in situ click chemistry to develop a cocktail of peptide-based capture agents, the components of which were raised against different, representative anti-HIV antibodies that bind to a conserved epitope of the HIV-1 envelope protein gp41. The cocktail was used to detect anti-HIV-1 antibodies from a panel of sera collected from HIV-positive patients, with improved signal-to-noise ratio relative to the gold standard commercial recombinant protein antigen. The capture agents were stable when stored as a powder for two months at temperatures close to 60°C.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Capture agent, PCC agent, In situ click chemistry, library screening
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Heath, James R.
Thesis Committee:
  • Grubbs, Robert H. (chair)
  • Clemons, William M.
  • Okumura, Mitchio
  • Heath, James R.
Defense Date:2 December 2013
Funders:
Funding AgencyGrant Number
Bill and Melinda Gates FoundationUNSPECIFIED
Institute for Collaborative BiotechnologiesW911NF-­09-­0001
Beckman Insitute pilot program "Spin-­Polarized Molecules for Structural and Systems Biology"UNSPECIFIED
Record Number:CaltechTHESIS:05262014-170142267
Persistent URL:http://resolver.caltech.edu/CaltechTHESIS:05262014-170142267
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1371/journal.pone.0076224DOIArticle adapted for ch. 2-3
http://dx.doi.org/10.1016/j.jmr.2010.04.010DOIArticle adapted for ch. 4
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8395
Collection:CaltechTHESIS
Deposited By: Jessica Pfeilsticker
Deposited On:29 May 2014 21:58
Last Modified:29 May 2014 21:58

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