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An in vivo Approach to tRNA Identity


Normanly, Jennifer (1989) An in vivo Approach to tRNA Identity. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/s9xt-6f20.


A leucine-inserting tRNA has been transformed into a serine-inserting tRNA by changing 12 nucleotides. Only 8 of the 12 changes are required to effect the conversion of the leucine tRNA to serine tRNA identity. The 8 essential changes reside in basepair 11-24 in the D stem, basepairs 3-70, 2-71 and nucleotides 72 and 73, all of the acceptor stem.

Functional amber suppressor tRNA genes were generated for 14 species of tRNA in E. coli, and their amino acid specificities determined. The suppressors can be classified into three groups, based upon their specificities. Class I suppressors, tRNAAla2CUA, tRNAGlyUCUA, tRNAHisACUA, tRNALysCUA, and tRNAProHCUA, inserted the predicted amino acid. The Class II suppressors, tRNAGluACUA, tRNAGlyTCUA, and tRNAIle1CUA were either partially or predominantly mischarged by the glutamine aminoacyl tRNA synthetase (AAS). The Class III suppressors, tRNAArgCUA, tRNAAspMCUA, tRNAIle2CUA, tRNAThr2CUA, tRNAMet(m)CUA and tRNAValCUA inserted predominantly lysine.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Biology
Degree Grantor:California Institute of Technology
Major Option:Biology
Awards:Lawrence L. and Audrey W. Ferguson Prize, 1989
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Abelson, John N.
Thesis Committee:
  • Abelson, John N. (chair)
  • Dervan, Peter B.
  • Emr, Scott D.
  • Richards, John H.
  • Simon, Melvin I.
Defense Date:20 December 1988
Record Number:CaltechTHESIS:06032013-145407392
Persistent URL:
Related URLs:
URLURL TypeDescription adapted for Introduction. adapted for Chapter I. adapted for Chapter III.
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7826
Deposited By: Benjamin Perez
Deposited On:03 Jun 2013 22:23
Last Modified:17 Sep 2021 20:53

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