An in vivo Approach to tRNA Identity

Citation

Normanly, Jennifer (1989) An in vivo Approach to tRNA Identity. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/s9xt-6f20. https://resolver.caltech.edu/CaltechTHESIS:06032013-145407392

Abstract

A leucine-inserting tRNA has been transformed into a serine-inserting tRNA by changing 12 nucleotides. Only 8 of the 12 changes are required to effect the conversion of the leucine tRNA to serine tRNA identity. The 8 essential changes reside in basepair 11-24 in the D stem, basepairs 3-70, 2-71 and nucleotides 72 and 73, all of the acceptor stem.

Functional amber suppressor tRNA genes were generated for 14 species of tRNA in E. coli, and their amino acid specificities determined. The suppressors can be classified into three groups, based upon their specificities. Class I suppressors, tRNA^Ala2_CUA, tRNA^GlyU_CUA, tRNA^HisA_CUA, tRNA^Lys_CUA, and tRNA^ProH_CUA, inserted the predicted amino acid. The Class II suppressors, tRNA^GluA_CUA, tRNA^GlyT_CUA, and tRNA^Ile1_CUA were either partially or predominantly mischarged by the glutamine aminoacyl tRNA synthetase (AAS). The Class III suppressors, tRNA^Arg_CUA, tRNA^AspM_CUA, tRNA^Ile2_CUA, tRNA^Thr2_CUA, tRNA^Met(m)_CUA and tRNA^Val_CUA inserted predominantly lysine.

Item Type:

Thesis (Dissertation (Ph.D.))

Subject Keywords:

Biology

Degree Grantor:

California Institute of Technology

Division:

Biology

Major Option:

Biology

Awards:

Lawrence L. and Audrey W. Ferguson Prize, 1989

Thesis Availability:

Public (worldwide access)

Research Advisor(s):

Abelson, John N.

Thesis Committee:

Abelson, John N. (chair)
Dervan, Peter B.
Emr, Scott D.
Richards, John H.
Simon, Melvin I.

Defense Date:

20 December 1988

Record Number:

CaltechTHESIS:06032013-145407392

Persistent URL:

https://resolver.caltech.edu/CaltechTHESIS:06032013-145407392

DOI:

10.7907/s9xt-6f20

Related URLs:

URL	URL Type	Description
https://doi.org/10.1146/annurev.bi.58.070189.005121	DOI	Article adapted for Introduction.
https://doi.org/10.1038/321213a0	DOI	Article adapted for Chapter I.
https://doi.org/10.1073/pnas.83.17.6548	DOI	Article adapted for Chapter III.

Default Usage Policy:

No commercial reproduction, distribution, display or performance rights in this work are provided.

ID Code:

7826

Collection:

CaltechTHESIS

Deposited By:

Benjamin Perez

Deposited On:

03 Jun 2013 22:23

Last Modified:

17 Sep 2021 20:53

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