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Synthesis and Biological Studies of DNA-binding Cyclic Py-Im Polyamides

Citation

Li, Benjamin Chun Yeung (2013) Synthesis and Biological Studies of DNA-binding Cyclic Py-Im Polyamides. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/82YX-QB29. https://resolver.caltech.edu/CaltechTHESIS:02282013-171348639

Abstract

Pyrrole-imidazole (Py-Im) polyamides are programmable oligomers that bind to the minor groove of DNA in a sequence-specific manner at affinities comparable to natural DNA-binding proteins. Hairpin polyamides have been shown to localize within the nucleus of live cells, disrupt protein-DNA interactions, and modulate endogenous gene expression. Cyclic polyamides display further enhanced DNA binding affinities and exhibit similar gene regulatory effects, but investigations into their biological activity have been limited by the lack of effective synthetic methods. Herein, we demonstrate the efficient synthesis of a focused library of cyclic polyamide utilizing a novel microwave-assisted solid-phase technique. The orthogonal protection strategy allowed for selective turn modifications, and the mild cleavage conditions gave access to polyamide cores beginning with a C-terminal imidazole. In addition to expanding our synthetic repertoire, we further examined the cytotoxicity and cell uptake profiles of the cyclic polyamide variants, which highlighted the significant changes in biological activity resulting from minor structural modifications. Molecular recognition of the polyamide turn unit was also explored by installing heteroatom substituents at the α-position. Interestingly, while none of the fluoro, hydroxyl, or amino derivatives increased turn specificity, the (S)-fluoro turn exhibited better tolerance for binding a C•G pair. Finally, we optimized the synthesis of several biologically active hairpin polyamides on a 50-mg scale and examined their antitumor activity in mice xenograft models.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:DNA; Py-Im Polyamide; Macrocycles; Microwave-Assisted; Solid-Phase Synthesis; Transcription Regulation; Anticancer
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Awards:Tobacco-Related Disease Research Program (TRDRP) Dissertation Award, 2009
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Dervan, Peter B.
Thesis Committee:
  • Tirrell, David A. (chair)
  • Stoltz, Brian M.
  • Rees, Douglas C.
  • Dervan, Peter B.
Defense Date:27 February 2013
Funders:
Funding AgencyGrant Number
NIHR01 GM-51747
TRDRP18DT-0015
Record Number:CaltechTHESIS:02282013-171348639
Persistent URL:https://resolver.caltech.edu/CaltechTHESIS:02282013-171348639
DOI:10.7907/82YX-QB29
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/jo302053vDOIUNSPECIFIED
http://dx.doi.org/10.1016/j.bmcl.2009.03.072DOIUNSPECIFIED
http://dx.doi.org/10.1007/s00280-012-1954-3DOIUNSPECIFIED
http://dx.doi.org/10.1073/pnas.1222035110 DOIUNSPECIFIED
http://dx.doi.org/10.1158/1535-7163.MCT-12-1040 DOIUNSPECIFIED
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7496
Collection:CaltechTHESIS
Deposited By: Benjamin Li
Deposited On:07 May 2013 21:24
Last Modified:03 Oct 2019 23:59

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