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Sequence specific effects on the incorporation of dideoxynucleotides by a modified T7 polymerase


Blanchard, Alan-Philippe (1993) Sequence specific effects on the incorporation of dideoxynucleotides by a modified T7 polymerase. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/9vz4-0e68.


While incorporating nucleotides onto the end of a DNA molecule, DNA polymerases selectively discriminate against dideoxynucleotides in favor of incorporating deoxynucleotides. The magnitude of this discrimination is modulated by the template DNA sequence near the incorporation site. This effect has been characterized by analyzing the raw data from a large number of DNA sequencing experiments. It is shown that, for bacteriophage T7 polymerase, the 5 contiguous bases extending from 3 bases 3' (on the template strand) from the incorporation site to 1 base 5' of the incorporation site are the most important in modulating dideoxynucelotide discrimination. A table of discrimination ratios for 1007 different 5-mer contexts is presented.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Computation and Neural Systems
Degree Grantor:California Institute of Technology
Major Option:Computation and Neural Systems
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Abu-Mostafa, Yaser S.
Thesis Committee:
  • Kajiya, James Thomas
  • Hopfield, John J.
  • Hood, Leroy E.
Defense Date:17 February 1993
Record Number:CaltechTHESIS:11122012-105407501
Persistent URL:
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7265
Deposited By: Dan Anguka
Deposited On:13 Nov 2012 15:59
Last Modified:09 Nov 2022 19:20

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