Citation
Wolf, John P. (1959) I. The alpha-chymotrypsin-catalyzed and base-catalyzed hydrolysis of some substituted glycine esters and related compounds. II. The nature and properties of the enzyme-substrate complex produced under conditions of very high substrate concentrations. III. A hypothesis concerning alpha-chymotrypsin-substrate complexes. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/KPJF-0G56. https://resolver.caltech.edu/CaltechETD:etd-02212006-134559
Abstract
A series of substituted glycogen esters have been evaluated as substrates for [alpha]-chymotrypsin. The second order base-catalyzed hydrolysis constants for these compounds have also been determined.
Four substrates for [alpha]-chymotrypsin have been found to exhibit unusual kinetic behavior at very high concentrations in the presence of [alpha]-chymotrypsin. It has been shown that these data can be the result of two molecules of substrate combining with each enzyme active site.
A hypothesis concerning the enzyme-substrate complex is offered which helps to correlate the data given in this work.
Item Type: | Thesis (Dissertation (Ph.D.)) |
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Degree Grantor: | California Institute of Technology |
Division: | Chemistry and Chemical Engineering |
Major Option: | Chemistry |
Thesis Availability: | Public (worldwide access) |
Research Advisor(s): |
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Thesis Committee: |
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Defense Date: | 1 January 1959 |
Record Number: | CaltechETD:etd-02212006-134559 |
Persistent URL: | https://resolver.caltech.edu/CaltechETD:etd-02212006-134559 |
DOI: | 10.7907/KPJF-0G56 |
Default Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. |
ID Code: | 694 |
Collection: | CaltechTHESIS |
Deposited By: | Imported from ETD-db |
Deposited On: | 01 Mar 2006 |
Last Modified: | 21 Dec 2019 03:56 |
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