I. The Alpha-Chymotrypsin-Catalyzed and Base-Catalyzed Hydrolysis of Some Substituted Glycine Esters and Related Compounds. II. The Nature and Properties of the Enzyme-Substrate Complex Produced under Conditions of Very High Substrate Concentrations. III. A Hypothesis Concerning Alpha-Chymotrypsin-Substrate Complexes

Citation

Wolf, John Peter (1959) I. The Alpha-Chymotrypsin-Catalyzed and Base-Catalyzed Hydrolysis of Some Substituted Glycine Esters and Related Compounds. II. The Nature and Properties of the Enzyme-Substrate Complex Produced under Conditions of Very High Substrate Concentrations. III. A Hypothesis Concerning Alpha-Chymotrypsin-Substrate Complexes. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/KPJF-0G56. https://resolver.caltech.edu/CaltechETD:etd-02212006-134559

Abstract

A series of substituted glycogen esters have been evaluated as substrates for [alpha]-chymotrypsin. The second order base-catalyzed hydrolysis constants for these compounds have also been determined. Four substrates for [alpha]-chymotrypsin have been found to exhibit unusual kinetic behavior at very high concentrations in the presence of [alpha]-chymotrypsin. It has been shown that these data can be the result of two molecules of substrate combining with each enzyme active site. A hypothesis concerning the enzyme-substrate complex is offered which helps to correlate the data given in this work.

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