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Cell migration domains in the chick telencephalon


Montgomery, John Michael (1996) Cell migration domains in the chick telencephalon. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/G3NH-0V88.


Little is known about the process by which the vertebrate forebrain (the diencephalon and telencephalon) becomes regionalized during development. In studies reported here, DiI injections were used to label the embryonic day 3 (stage-16) chick telencephalon in ovo , and the migration patterns of labelled cells were analyzed in relation to various molecular markers, including the regulatory genes Cash-l and Sonic hedgehog (Shh). Cells generated in the ventral telencephalon (basal ventricular ridge, or BVR) were found to migrate widely, but were restricted from crossing into the more dorsal telencephalon (dorsal ventricular ridge, or DVR), and the more caudal diencephalon. The cell migration boundary between the BVR and DVR correlates with a Cash-l expression boundary, and the cell migration boundary between BVR and diencephalon correlates with a Shh expression boundary. In addition, cell migration patterns were dramatically different in the BVR and DVR territories. These results suggest that the BVR represents a "cell migration domain," or a true unit of telencephalic compartmental organization, which is distinct from cell migration domains in both the DVR and diencephalon. In addition, two lines of evidence in the early embryo are shown to support the proposal that avian BVR is homologous to mammalian basal ganglia, and that avian DVR is homologous to mammalian cerebral cortex: regulatory gene expression patterns in the chick and mouse telencephalon are very similar; and the cell migration patterns in the chick telencephalon demonstrated here are found to correspond closely to those previously reported in the mouse telencephalon. Using the same DiI labelling technique, a regional fate map of the stage-16 chick telencephalon was derived. This fate map can now be used to guide transplantation or misexpression experiments, and to interpret gene expression patterns in the stage-16 telencephalon. For example, though Cash-l is expressed in the entire BVR at ES-E7 (stage 24-30), superimposition of its expression pattern onto the stage-16 fate map shows that it is only expressed in a subregion of the presumptive BVR at stage-16.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Cellular and Molecular Neurobiology
Degree Grantor:California Institute of Technology
Major Option:Biology
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Anderson, David J. (advisor)
  • Fraser, Scott E. (advisor)
Thesis Committee:
  • Unknown, Unknown
Defense Date:24 January 1996
Record Number:CaltechTHESIS:06022011-144716111
Persistent URL:
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6491
Deposited On:02 Jun 2011 22:50
Last Modified:08 Nov 2023 00:22

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