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Studies on the Feasibility of Isolating the Acetyl Choline Receptor by Means of Affinity Chromatography


Erb, Judith Louise (1971) Studies on the Feasibility of Isolating the Acetyl Choline Receptor by Means of Affinity Chromatography. Master's thesis, California Institute of Technology. doi:10.7907/RBRP-RY38.


Feasibility studies were conducted on the isolation of the acetyl choline receptor by means of affinity chromatography. Sepharose resins bearing acetyl choline receptor binding materials of at various distances from the backbone of the resin and of varying degrees of substitution were treated with extracts from electric eel organ, washed free of non-binding materials and the material bound to the column extracted by various means. Extracts from the column were studied for purity, binding to decamethonium and α-bungarotoxin, and presence or absence of acetyl cholinesterase or choline acetylase activity. Although material possessing the proper characteristics was extracted from some of the resins, electrophoretic purity was not obtained.

Item Type:Thesis (Master's thesis)
Subject Keywords:(Chemistry)
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Raftery, Michael A.
Thesis Committee:
  • Unknown, Unknown
Defense Date:21 May 1971
Record Number:CaltechTHESIS:04292010-151021376
Persistent URL:
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:5758
Deposited By: Tony Diaz
Deposited On:08 Jul 2010 17:36
Last Modified:18 Jul 2023 23:05

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