Studies on the Feasibility of Isolating the Acetyl Choline Receptor by Means of Affinity Chromatography

Citation

Erb, Judith Louise (1971) Studies on the Feasibility of Isolating the Acetyl Choline Receptor by Means of Affinity Chromatography. Master's thesis, California Institute of Technology. doi:10.7907/RBRP-RY38. https://resolver.caltech.edu/CaltechTHESIS:04292010-151021376

Abstract

Feasibility studies were conducted on the isolation of the acetyl choline receptor by means of affinity chromatography. Sepharose resins bearing acetyl choline receptor binding materials of at various distances from the backbone of the resin and of varying degrees of substitution were treated with extracts from electric eel organ, washed free of non-binding materials and the material bound to the column extracted by various means. Extracts from the column were studied for purity, binding to decamethonium and α-bungarotoxin, and presence or absence of acetyl cholinesterase or choline acetylase activity. Although material possessing the proper characteristics was extracted from some of the resins, electrophoretic purity was not obtained.

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