Citation
Wallace, Frederic Andrew (1961) pH Titration Studies of the Acid Denaturation of Calf Thymus Deoxyribosenucleic Acid. Master's thesis, California Institute of Technology. doi:10.7907/MME6-8038. https://resolver.caltech.edu/CaltechTHESIS:04212010-142614826
Abstract
The acid denaturation of calf thymus deoxyribosenucleic acid (DNA) has been studied both by PH and spectrophotometric titration in the temperature range 10 - 30°C and at ionic strengths 0.1 F and 0.5 F. The number of hydrogen ions bound by the DNA at any given pH increased with increasing temperature and decreased with increasing ionic strength. The number bound at any given pH was greater after denaturation. A model for acid denaturation is proposed. The site of protonation of cytosine is discussed.
| Item Type: | Thesis (Master's thesis) |
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| Subject Keywords: | (Chemistry) |
| Degree Grantor: | California Institute of Technology |
| Division: | Chemistry and Chemical Engineering |
| Major Option: | Chemistry |
| Thesis Availability: | Public (worldwide access) |
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| Thesis Committee: |
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| Defense Date: | 1960 |
| Record Number: | CaltechTHESIS:04212010-142614826 |
| Persistent URL: | https://resolver.caltech.edu/CaltechTHESIS:04212010-142614826 |
| DOI: | 10.7907/MME6-8038 |
| Default Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. |
| ID Code: | 5730 |
| Collection: | CaltechTHESIS |
| Deposited By: | Tony Diaz |
| Deposited On: | 21 Jun 2010 18:24 |
| Last Modified: | 09 Nov 2023 20:09 |
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