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A Genetic Analysis of Bacteriophage Lambda


Kaiser, Armin Dale (1955) A Genetic Analysis of Bacteriophage Lambda. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/RR9B-8753.


The genetic structure of the temperate bacteriophage lambda was analysed by means of crosses. Mutations in lambda which affected the size or type of plaque initiated by single particles were induced by ultraviolet irradiation. Each of four mutants so obtained was found to differ by one factor from the reference type. Two factor crosses demonstrated the existence of linkage between the 4 mutant factors and suggested a linear sequence which was later confirmed by three factor crosses. In addition 5 other factors were found to be linked to the first 4, thus all 9 factors studied seem to lie on the same linkage group. A striking feature of three factor crosses was the high frequency of double crossover types. This "apparent negative interference" was traced to the yields of individual bacteria by means of single burst experiments. Finally, the compatibility of the results of the lambda crosses with two different theories of phage recombination was examined.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:(Biophysics and Chemistry)
Degree Grantor:California Institute of Technology
Major Option:Biology
Minor Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Weigle, Jean J.
Thesis Committee:
  • Unknown, Unknown
Defense Date:1 January 1955
Record Number:CaltechETD:etd-12032003-105441
Persistent URL:
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4736
Deposited By: Imported from ETD-db
Deposited On:10 Dec 2003
Last Modified:29 Jun 2023 22:14

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PDF (Kaiser_ad_1955.pdf) - Final Version
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