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Neuron-Microdevice Connections


Regehr, Wade Gordon (1988) Neuron-Microdevice Connections. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/nshf-ww49.


A new method for long-term recording and stimulation applicable to cultured neurons has been developed. Silicon-based microelectrodes have been fabricated using integrated-circuit technology and micromachining. The chronic connection is made by positioning the electrode tip into contact with the cell body, and gluing the device to the bottom of the culture dish. These "diving-board electrodes" consist of an insulated lead exposed only at the tip sealed to the cell body of a cultured neuron. A two-way electrical connection to Helisoma B19 neurons has been established for up to four days. Preliminary experiments with cultured superior cervical ganglion neurons indicate diving-board electrodes can be used with cultured neurons larger than 20 µm in diameter.

In a related technique Helisoma neurons grown on special dish containing a multielectrode array were found to seal to the dish electrodes, establishing similar long-term connections. This capability will make it possible to conduct experiments with either diving-board electrodes or dishes that cannot be performed using conventional techniques.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Applied Physics
Degree Grantor:California Institute of Technology
Division:Engineering and Applied Science
Major Option:Applied Physics
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Rutledge, David B.
Thesis Committee:
  • Rutledge, David B. (chair)
  • Bellan, Paul Murray
  • Lester, Henry A.
  • Bower, James M.
  • Pine, Jerome
Defense Date:25 March 1988
Record Number:CaltechETD:etd-11092007-084226
Persistent URL:
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4478
Deposited By: Imported from ETD-db
Deposited On:05 Dec 2007
Last Modified:16 Apr 2021 23:16

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