Part I. α-Secondary Isotope Effects on the Reaction Catalyzed by the Enzyme Fumarase. Part II. Salt Effects on the Enzyme Fumarase

Citation

Schmidt, Donald Emil, Jr. (1967) Part I. α-Secondary Isotope Effects on the Reaction Catalyzed by the Enzyme Fumarase. Part II. Salt Effects on the Enzyme Fumarase. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/D85P-2N72. https://resolver.caltech.edu/CaltechETD:etd-10042002-125501

Abstract

NOTE: Text or symbols not renderable in plain ASCII are indicated by [...]. Abstract is included in .pdf document. PART I The [alpha]-secondary isotope effect of L-malic-2-T-1, 4-C[superscript 14] acid on the reaction catalyzed by fumarase was determined. Evidence was presented which indicated that the reaction proceeded by formation of a carbonium ion in a rate-determining state at C-2 which possesses little sp[superscript 2] character. PART II The effects of sodium bromide and sodium phosphate at pH 7.3 and 27[degrees] were determined. A partial kinetic and mechanistic scheme has been developed to explain these effects.

Thesis Files