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Studies on the Interaction of Subviral Particles of the Bacteriophage ΦX174 with Protoplasts of Escherichia coli


Guthrie, George Drake (1962) Studies on the Interaction of Subviral Particles of the Bacteriophage ΦX174 with Protoplasts of Escherichia coli. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/197P-A064.


Interaction of Subviral Particles of Bacteriophage [phi]X174 with Protoplasts of Escherichia coli. Part I: Infection of Protoplasts of Escherichia coli by Subviral Particles of Bacteriophage [phi]X174. G. D. Guthrie and R. L. Sinsheimer, J. Mol. Biol., (1960), 2, 297-305. Infection of protoplasts of E. coli leading to the production of mature phage can occur with subviral particles of phage [phi]X174, including the purified DNA of the phage. Methods for obtaining the proper conditions for infection are described. Results of experiments describing the characteristics of the infection and the nature of the subviral particles causing the infection are presented. Evidence is presented that net synthesis of mature [phi]X174 can be obtained from infection of protoplasts by purified [phi]X174 DNA. Part II: Initial Studies on Protoplasts and [phi]X-DNA as a system for investigating virus replication. G. D. Guthrie (unpublished thesis research). Further investigations on the interaction of [phi]X-DNA with protoplasts are described. General techniques to be employed in using the system for the study of the replication of virus are outlined. Data from investigations on the initial "infection" process of [phi]X-DNA are presented. From these data, factors influencing the level of infection are discussed and a simple mathematical relationship relating these parameters to the infection is proposed.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:(Biophysics)
Degree Grantor:California Institute of Technology
Major Option:Biology
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Sinsheimer, Robert L.
Thesis Committee:
  • Unknown, Unknown
Defense Date:1 January 1962
Record Number:CaltechETD:etd-08092006-095212
Persistent URL:
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3068
Deposited By: Imported from ETD-db
Deposited On:09 Aug 2006
Last Modified:22 Nov 2023 21:56

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PDF (Guthrie_gd_1962.pdf) - Final Version
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