Citation
Kurtz, Abraham Nathan (1960) I. Structural Analogs of Typical Substrates of Alpha-Chymotrypsin. II. l-Acetyl-2-[L-Tyrosyl] Hydrazine: an Inhibitor of Alpha-Chymotrypsin. Ill. Binuclear Aromatics as Inhibitors of Alpha-Chymotrypsin Catalyzed Hydrolyses. IV. Applicability of the pH-Stat to Alpha-Chymotrypsin Catalyzed Hydrolyses that Produce a Buffer. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/8C7N-4Z18. https://resolver.caltech.edu/CaltechETD:etd-06302006-085322
Abstract
Substitution of a nitrogen atom in place of the C-H group that occurs at the asymmetric center of typical substrates of alpha-chymotrypsin results in the complete loss of the ability of the enzyme to catalyze the hydrolysis of the carboethoxy or carboxamido group. However, the nitrogen-substituted analogs, which otherwise possess all of the remaining significant structural entities of typical substrates, function as inhibitors of alpha-chymotrypsin catalyzed hydrolyses but in general show less affinity for the enzyme than do the analogous C-H containing substrates. It has been found that l-acetyl-2-[L-tyrosyl] hydrazine is a reversible inhibitor of alpha-chymotrypsin catalyzed hydrolyses with unusual affinity for the enzyme. At pH 7.9 the enzyme inhibitor constant was found to have a value of KI = 0.074 x 10(-3)M, which shows that this substance associates reversibly with alpha-chymotrypsin at the active site to a greater extent than any other known substrate or reversible inhibitor. Preliminary results, based upon inhibition studies, indicate that alpha-chymotrypsin possesses a greater affinity for the naphthalene function than for the indole function. A study of the applicability of the pH-stat to alpha-chymotrypsin catalyzed hydrolyses that produce a buffer showed that the reliability was a function of the buffer capacity. Amides gave poor results; hydrazides gave good or fair results, one of which contradicted a literature value, and a hydroxamide gave excellent results.
Item Type: | Thesis (Dissertation (Ph.D.)) |
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Subject Keywords: | (Chemistry) |
Degree Grantor: | California Institute of Technology |
Division: | Chemistry and Chemical Engineering |
Major Option: | Chemistry |
Thesis Availability: | Public (worldwide access) |
Research Advisor(s): |
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Thesis Committee: |
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Defense Date: | 1 January 1960 |
Record Number: | CaltechETD:etd-06302006-085322 |
Persistent URL: | https://resolver.caltech.edu/CaltechETD:etd-06302006-085322 |
DOI: | 10.7907/8C7N-4Z18 |
Default Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. |
ID Code: | 2789 |
Collection: | CaltechTHESIS |
Deposited By: | Imported from ETD-db |
Deposited On: | 14 Jul 2006 |
Last Modified: | 07 Nov 2023 18:24 |
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