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Modification of Membrane Surfaces with Carbohydrates: An Approach for Stabilization During Freezing and Drying


Goodrich, Raymond Paul, Jr. (1990) Modification of Membrane Surfaces with Carbohydrates: An Approach for Stabilization During Freezing and Drying. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/ff9e-th51.


A new class of molecules possessing amphipathic character was prepared. These compounds possessed a hydrophobic region capable of intercalation into a lipid bilayer, a hydrophilic linker group capable of extending beyond the surface of a membrane, and a carbohydrate attached at the end of this linker group. These features of this class of compounds permitted their direct incorporation into vesicle formulations and hence the direct examination of interactions occuring in the dry state between carbohydrates and lipid groups in such vesicle membrane systems.

Samples of treated vesicle preparations were subjected to freezing and thawing as well as to direct dehydration via lyophilization. Under these conditions, the stability and integrity of the membrane was examined via several spectroscopic techniques.

Through these studies of systems in which a carbohydrate is directly bound to a membrane surface, it was possible to determine a defined ratio, independent of solution and concentration effects, at which carbohydrates can afford protection to dehydrated membranes. In addition, the interactions responsible for conferring the protection were determined. It was found that direct intercalation of the carbohydrates into a membrane interface preserves the membrane structure and organization that is normally observed in the presence of water. This behavior prevents the phase transitions, lipid phase separations, and fusion phenomena that normally compromise dehydrated membrane systems. This phenomena is directly related to the amount of carbohydrate that is present and the structure of the carbohydrate that is used. These results indicate that the partitioning behavior of the carbohydrates at the interface is of prime importance in determining the effectiveness in this regard.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Chemistry
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Baldeschwieler, John D.
Thesis Committee:
  • Grubbs, Robert H. (chair)
  • Baldeschwieler, John D.
  • Revel, Jean-Paul
  • Imperiali, Barbara
Defense Date:4 January 1990
Funding AgencyGrant Number
Record Number:CaltechETD:etd-06072007-091301
Persistent URL:
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2503
Deposited By: Imported from ETD-db
Deposited On:18 Jun 2007
Last Modified:11 Feb 2022 21:58

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