An Immunological Study of Chicken Serum Albumin and Related Organ Antigens

Citation

Rappaport, Irving (1953) An Immunological Study of Chicken Serum Albumin and Related Organ Antigens. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/CKW1-1Z19. https://resolver.caltech.edu/CaltechETD:etd-05082003-163307

Abstract

1. The percentage of the proteins in the plasma of New Hampshire Red chickens was found to increase from 2.0g to 5.89g per 100 ml., as the chickens matured. 2. The albumin/globulin ratio decreased from 0.75 to 0.25 with increasing age of the chickens. 3. The mobility of serum albumin in veronal buffer at pH 8.6, u= 0.1 was found to be 6.8 ? 0.11 x 10[superscript -5] cm[superscript 2]/volt/sec. 4. More than 95% of the soluble proteins from livers and kidneys migrated with mobilities different from serum albumin. 5. Precipitin tests suggested that the soluble proteins of the liver include approximately one-half of one per cent homologous serum albumin, whereas the kidney does not include any serologically detectable amounts of serum albumin. 6. No measurable cross reactions occurred between rabbit anti-chicken serum albumin and the soluble proteins from chicken livers and kidneys. 7. More serum albumin was detected after the incubation of liver slices in KCl metabolite than before incubation. This suggested that a net synthesis may have occurred. 8. More serum albumin was detected in the soluble proteins within the slices after incubation in KC1 metabolite to which different amounts of antiserum were added than in the controls where normal rabbit serum was present. This suggested that the antiserum inhibited the release of serum albumin from the cells. 9. The significance of these findings has been discussed.

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