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l. Some Studies in Enzyme Kinetics. II. The Oxidation of 3-Indoleacetic Acid by Plant Enzymes. III. The Synthesis of Some α-Alkyl α-Amino Acids and Their Derivatives

Citation

Manning, David Treadway (1955) l. Some Studies in Enzyme Kinetics. II. The Oxidation of 3-Indoleacetic Acid by Plant Enzymes. III. The Synthesis of Some α-Alkyl α-Amino Acids and Their Derivatives. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/PC3Z-A208. https://resolver.caltech.edu/CaltechETD:etd-01142004-142952

Abstract

NOTE: Text or symbols not renderable in plain ASCII are indicated by [...]. Abstract is included in .pdf document. The kinetics of the alpha-chymotrypsin catalyzed hydrolysis of N-carboethoxy-L-tyrosinamide have been determined in aqueous solution at 25[degrees]C and at both pH 7.9 and pH 8.2. The enzyme-inhibitor dissociation constants of N-carboethoxy-D-tyrosinamide, N-carboethoxy-D-tyrosinmethylamide, and N-carboethoxy-L-tyrosinmethylemide have been evaluated in aqueous solution at 25[degrees]C, the former at pH 7.9 and the latter two at pH 7.6. The L isomers of each enantiomorphic pair appear to have a greater affinity for the enzyme than do their respective D-isomers. An investigation into the nature of the enzymatic oxidation products of 3-Indoleacetic acid (IAA) has been made. 3-Indolealdehyde, o-formamidoacetophenone, o-aminoacetophenone, and 4-hydroxyquinoline have been eliminated as possible major products of the reaction. A study of synthetic routes to the alpha-alkyl alpha-amino acids has been made. DL-alpha-Methlphenylalanine and DL-alpha-methyltyrosine have been synthesized. All attempts to resolve these compounds by enzymatic means have failed.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:(Chemistry and Biology)
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Minor Option:Biology
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Niemann, Carl G.
Thesis Committee:
  • Unknown, Unknown
Defense Date:1 January 1955
Record Number:CaltechETD:etd-01142004-142952
Persistent URL:https://resolver.caltech.edu/CaltechETD:etd-01142004-142952
DOI:10.7907/PC3Z-A208
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:165
Collection:CaltechTHESIS
Deposited By: Imported from ETD-db
Deposited On:16 Jan 2004
Last Modified:06 Jul 2023 18:01

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