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Structural Analysis of MurG Interactions with Substrates, Inhibitors, and MraY


Brackney, Helen Maya (2024) Structural Analysis of MurG Interactions with Substrates, Inhibitors, and MraY. Senior thesis (Major), California Institute of Technology. doi:10.7907/y324-9f36.


The peptidoglycan layer in bacterial cells is a popular target for antibiotic development. The membrane protein MraY and peripheral membrane protein MurG are part of critical steps in the synthesis of peptidoglycan. Lipid I, a lipid precursor formed by MraY, is recognized by MurG through its soluble domain. Currently, there is no structure of MurG with bound Lipid I, and the residues required for this interaction have not been conclusively defined. Crystallographic methods and Cryo-Electron Microscopy were applied to study the interactions between MurG and the soluble domain of Lipid I by binding Park’s Nucleotide, Lipid II, or a Lipid I analog were used to study the interactions of MurG and MraY with the aforementioned substrates. By adding Park’s Nucleotide, Murgocil, Lipid II, the Lipid I analog, or a combination of the listed additives to concentrated MurG, crystals formed under optimized conditions. We aim to obtain electron-density maps from these techniques to model the structure of MurG.

Item Type:Thesis (Senior thesis (Major))
Subject Keywords:Peptidoglycan Biosynthesis Pathway, membrane protein, MurG, MraY, x-ray crystallography, nanodiscs,
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemical Engineering
Awards:John Stauffer Summer Undergraduate Research Fellowship 2023. John Stauffer Summer Undergraduate Research Fellowship 2022. Donald Voet and Jerome Vinograd Summer Undergraduate Research Fellowship 2021.
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Clemons, William M.
Thesis Committee:
  • None, None
Defense Date:24 August 2023
Non-Caltech Author Email:helenbrackney (AT)
Record Number:CaltechTHESIS:10242023-173046393
Persistent URL:
Brackney, Helen Maya0009-0009-8067-768X
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:16214
Deposited By: Helen Brackney
Deposited On:26 Oct 2023 19:23
Last Modified:08 Nov 2023 00:18

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