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Developing Technologies for Real-Time Whole-Organism Imaging via FTIR Spectromicroscopy


Holman, Elizabeth Anne (2022) Developing Technologies for Real-Time Whole-Organism Imaging via FTIR Spectromicroscopy. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/es4r-kq84.


FTIR spectromicroscopy heavily resides in the domain of cell-based and tissue-based studies when focusing on its direct application to biological systems. The goal of the reported graduate research is to extend FTIR spectromicroscopy to multicellular whole-organism imaging, ideally for non-invasive, non-destructive, and label-free spatiochemical imaging of biological model Caenorhabditis elegans (C. elegans). With modern optics, detector, and light source technologies implemented at synchrotron facilities, this thesis focuses on exploring the feasibility of multicellular whole-organism imaging while identifying challenges and presenting working solutions for them.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:FTIR, spectromicroscopy, infrared, chemical imaging, synchrotron, autonomous experimentation, peptoid, whole-organism
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Restricted to Caltech community only
Research Advisor(s):
  • Sternberg, Paul W. (advisor)
  • Rees, Douglas C. (co-advisor)
Thesis Committee:
  • Davis, Mark E. (chair)
  • Grubbs, Robert H.
  • Okumura, Mitchio
  • Sternberg, Paul W.
  • Rees, Douglas C.
Defense Date:6 July 2021
Non-Caltech Author Email:holman.ea (AT)
Funding AgencyGrant Number
National Science Foundation Graduate Research Fellowship1745301
Howard Hughes Medical Institute (HHMI)047-101
DOE Science Graduate Student Research (SCGSR) AwardUNSPECIFIED
Record Number:CaltechTHESIS:08312021-211824138
Persistent URL:
Related URLs:
URLURL TypeDescription in Communications Biology for work described in Chapter 3 used in work described in Chapter 3
Holman, Elizabeth Anne0000-0002-5158-4689
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:14350
Deposited By: Elizabeth Holman
Deposited On:07 Sep 2021 19:15
Last Modified:06 Jan 2023 18:48

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