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Multiscale Mechanical Characterization of Subcellular Structures in Living Walled Cells

Citation

Ginsberg, Leah Morgan (2021) Multiscale Mechanical Characterization of Subcellular Structures in Living Walled Cells. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/avj4-ve78. https://resolver.caltech.edu/CaltechTHESIS:03262021-224805539

Abstract

The physiology of walled cells is dramatically different from that of human cells, but the biomechanics of walled cells are far less studied. Most bacterial, fungal, and plant cells have a strong cell wall (CW), which allows them to withstand large hydrostatic pressures in the cytoplasm, called turgor. Turgor pressure conflates the mechanics of subcellular components and complicates the characterization of the cell. In this dissertation, new models are introduced and explored for single cells to investigate the multiscale mechanics of plant and bacterial cells using micro- and nano-indentation experiments.

A multi-scale biomechanical assay is used to study the mechanical properties of plant cells. The plant CW is typically around 5% of the width of the entire cell, and is thought to carry most of the mechanical load. Large-scale indentations using a micro-indentation system probe the behavior of the overall cell structure, and atomic-force microscopy (AFM) nano-scale indentations are used to isolate the CW response. To determine the effect of external osmotic pressure, indentations are performed on cells in different osmotic conditions: hypotonic, isotonic, and hypertonic. The cell is idealized as two springs acting in series, one to represent the CW and one to represent the cytoplasm. The model uses the experimentally determined initial stiffnesses as input to the model to determine the relative stiffness contributions of the CW and the cytoplasm.

The first type of walled cells investigated is the xylem vessel element of Arabidopsis thaliana. The xylem is responsible for transporting water through the stem of any vascular plant (more commonly known as a land plant), and hence it must maintain structural integrity against high internal pressures while transporting water from the roots to the leaves. For extra structural support, xylem vessel elements develop secondary cell walls (SCWs), which are known to be a key component for mediating mechanical strength and stiffness in vascular plants. The structure and biomechanics of cultured plant cells are investigated during the cellular developmental stages associated with SCW formation using the multi-scale biomechanical assay described above. To determine the effect of morphological changes during differentiation, micro- and nano-indentations are performed on cells in different observed stages of the differentiation process.Prior to triggering differentiation, cells in hypotonic pressure conditions are significantly stiffer than cells in isotonic or hypertonic conditions, highlighting the dominant role of turgor pressure. Plasmolyzed cells with a SCW reach similar levels of stiffness as cells with maximum turgor pressure. Analysis using the two-spring model shows that the stiffness of the primary CW in all of these conditions is lower than the stiffness of the fully-formed SCW. These results provide the first experimental characterization of the mechanics of SCW formation at the single-cell level in plant cells.

Next, the mechanical response of individual Nicotiana tabacum cells from a suspension culture is studied using the same multi-scale biomechanical assay. The role played by the microtubules (MTs) and actin filaments (AFs) is determined through the use of drug treatments which selectively remove MTs and AFs. A generative statistical model is added to the two-spring model to quantify the stiffnesses of the CW, cytoplasm, turgor pressure, MTs, and AFs. Analysis of the initial stiffness and energy dissipation calculated from micro-indentation experiments indicates that the MTs and AFs contribute significantly to the mechanical response of a cell under compression. Micro- and nano-indentation tests confirm that turgor pressure is the most significant contributor to the stiffness response of turgid cells in compression. Finally, the results reveal that turgor pressure exerts stress on the CW, which leads to a measurable stiffening of the CW.

The studies described above focused on developing a discrete model to describe the mechanics of a cell in indentation experiments. However, the most common type of model used to evaluate the mechanics of a cell are continuum models. Continuum models are also necessary to decouple the material properties of subcellular components from their structure. In the final section, AFM indentations are simulated on a gram-negative bacterium, Escherichia coli, and a sensitivity study and inverse analysis are performed to solve for the CW elastic modulus and turgor pressure simultaneously. Sensitivity study results reveal that uncertainty in turgor pressure and CW elasticity indeed contribute the most to variability in force spectra from AFM measurements. The parameter space of possible values for CW elastic modulus and turgor pressure is discretized using triangular elements. "Simulated experiments" are tested throughout the parameter space, and correlations between the CW elastic modulus and turgor pressure, which depend on the type of objective function, are investigated. Two unique objective functions are tested in the inverse analysis, and a third objective function, which is a weighted sum of the first two, is found to reduce errors in estimated CW elastic modulus and turgor pressure by 20% and 11%, respectively. The use of this type of inverse analysis has the potential to elucidate the material properties of CWs using a single indentation measurement and reliably decouple these properties from the high turgor pressures inside walled cells.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Arabidopsis thaliana; atomic-force microscopy (AFM); cell wall; cytoskeleton; Escherichia coli; micro-indentation; nano-indentation; Nicotiana tabacum; plant biomechanics; turgor pressure; micro-compression; statistical modeling
Degree Grantor:California Institute of Technology
Division:Engineering and Applied Science
Major Option:Mechanical Engineering
Thesis Availability:Restricted to Caltech community only
Research Advisor(s):
  • Ravichandran, Guruswami
Thesis Committee:
  • Bhattacharya, Kaushik
  • Daraio, Chiara (chair)
  • Ravichandran, Guruswami
  • Roumeli, Eleftheria
Defense Date:15 March 2021
Non-Caltech Author Email:leah.m.ginsberg (AT) gmail.com
Funders:
Funding AgencyGrant Number
Defense Advanced Research Projects Agency (DARPA)HR0011-17-2-0037
Record Number:CaltechTHESIS:03262021-224805539
Persistent URL:https://resolver.caltech.edu/CaltechTHESIS:03262021-224805539
DOI:10.7907/avj4-ve78
Related URLs:
URLURL TypeDescription
https://doi.org/10.3390/plants9121715DOIPublished version of Chapter 2 in Plants Journal.
ORCID:
AuthorORCID
Ginsberg, Leah Morgan0000-0001-9685-7014
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:14112
Collection:CaltechTHESIS
Deposited By: Leah Ginsberg
Deposited On:15 Apr 2021 17:01
Last Modified:15 Apr 2021 17:01

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