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Facilitating Miniaturized Bioanalytical Assays in Microfluidic Devices

Citation

Zhukov, Dmitriy Vladimirovich (2020) Facilitating Miniaturized Bioanalytical Assays in Microfluidic Devices. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/x9wq-h920. https://resolver.caltech.edu/CaltechTHESIS:02062020-145031701

Abstract

This work describes several efforts in making microfluidic lab-on-a-chip technology more convenient to use for bioanalysis in limited-resource settings (Chapters 2-3), and describes a device for miniaturized multistep process execution (Chapter 4). One underlying theme of these projects is the streamlining of the 'chip-to-world' interfacing to help bring this technology from specialized labs of its developers into more widespread utilization by potential users in other disciplines. Chapter 2 outlines a portable method for achieving stable fluid pumping for sample loading and flow control in microfluidic devices. Chapter 3 details a method for digital nucleic acid test readout with unmodified smartphone cameras. Chapter 4 demonstrates a lab-on-a-chip platform capable of carrying out complex multiplexed biochemical reactions requiring multiple sequential additions of reagents by performing RNA barcoding for multiplexed cDNA library generation.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Microfluidics; lab-on-a-chip; pressure driven flow control; digital nucleic acid amplification test; cellphone camera readout; colorimetric readout; SlipChip; assay miniaturization; multistep biochemistry; droplets; droplet merging; RNA-seq
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemical Engineering
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Ismagilov, Rustem F.
Thesis Committee:
  • Tirrell, David A. (chair)
  • Ismagilov, Rustem F.
  • Guttman, Mitchell
  • Gao, Wei
Defense Date:13 December 2019
Non-Caltech Author Email:dvzhukov (AT) outlook.com
Funders:
Funding AgencyGrant Number
NSF Graduate Research FellowshipDGE-1144469
Rosen Center for Bioengineering at CaltechUNSPECIFIED
Record Number:CaltechTHESIS:02062020-145031701
Persistent URL:https://resolver.caltech.edu/CaltechTHESIS:02062020-145031701
DOI:10.7907/x9wq-h920
Related URLs:
URLURL TypeDescription
https://doi.org/10.1039/C4LC00910JDOIArticle adapted for Chapter 2.
https://pubs.acs.org/doi/10.1021/acsnano.5b07338DOIArticle adapted for Chapter 3.
https://doi.org/10.1039/C9LC00541BDOIArticle adapted for Chapter 4.
ORCID:
AuthorORCID
Zhukov, Dmitriy Vladimirovich0000-0002-4834-3147
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:13636
Collection:CaltechTHESIS
Deposited By: Dmitriy Zhukov
Deposited On:21 May 2020 16:43
Last Modified:17 Jun 2020 19:46

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