Citation
Chiang, Huey-jenn (1987) Studies of the Thermodynamics of Some Native and Ruthenated Metalloproteins by Spectroelectrochemistry. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/8fcf-kt72. https://resolver.caltech.edu/CaltechTHESIS:10312019-170145504
Abstract
The thermodynamic parameters of redox centers in some metalloproteins have been investigated using spectroelectrochemical techniques, with the employment of an OTTLE (Optically Transparent Thin-Layer Electrode) cell. With the aid of various carefully selected mediators, the temperature dependence of the formal redox potentials of type I copper (blue copper) ions in Rhus vernicifera (tree) laccase, as well as in native and in pentaammineruthenium-modified azurins from Pseudomonas aeruginosa, have been measured. Similar experiments have been carried out for the heme site of cis-[Ru(en)2(OH)(His)]-horse heart cytochrome c (en: ethylenediamine; His: histidine). Cyclic voltammetry has been used to study the pH-dependence of the formal redox potentials of the appended ruthenium ion in cis-[Ru(en)2(OH)(His)]-horse heart cytochrome c. Finally, the synthesis and spectroelectrochemistry of sperm whale myoglobin reconstituted with [Ru(MpIX)(DMSO) dicarboxylic acid]- moiety (MpIX: mesoporphyrin IX; DMSO: dimethyl sulfoxide) have also been carried out.
| Item Type: | Thesis (Dissertation (Ph.D.)) |
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| Subject Keywords: | Chemistry |
| Degree Grantor: | California Institute of Technology |
| Division: | Chemistry and Chemical Engineering |
| Major Option: | Chemistry |
| Thesis Availability: | Public (worldwide access) |
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| Thesis Committee: |
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| Defense Date: | 4 May 1987 |
| Record Number: | CaltechTHESIS:10312019-170145504 |
| Persistent URL: | https://resolver.caltech.edu/CaltechTHESIS:10312019-170145504 |
| DOI: | 10.7907/8fcf-kt72 |
| Default Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. |
| ID Code: | 11878 |
| Collection: | CaltechTHESIS |
| Deposited By: | Mel Ray |
| Deposited On: | 01 Nov 2019 00:25 |
| Last Modified: | 16 Apr 2021 23:05 |
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