Citation
Mosesso, Richard Albert (2019) Mechanistic Investigations of Receptor Signaling via Canonical and Non-Canonical Amino Acid Mutagenesis. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/X18Z-XE16. https://resolver.caltech.edu/CaltechTHESIS:06072019-131615199
Abstract
This dissertation primarily describes investigations of the mechanisms by which pentameric ligand-gated ion channels (pLGICs) activate ("gating") using canonical and non-canonical amino acid mutagenesis. Chapter 1 provides an introduction to the systems being studied, their physiological roles, and the techniques that we have used to study them. Chapter 2 describes a series of experiments comparing the roles of amino acid residues proximal to the neurotransmitter binding site in the type 3 serotonin receptor (5-HT3R) to the aligning residues of the muscle-type nicotinic acetylcholine receptor (nAChR). The findings presented in Chapter 3 assess the functional roles of proline residues in the prokaryotic pLGIC, Erwinia ligand-gated ion channel (ELIC). Chapter 4 describes an extensive investigation of salient details of 5-HT3R gating using canonical and non-canonical amino acid mutagenesis of amino acid residues at the interface of the extracellular domain and transmembrane domain of this receptor. Chapter 5 applies a photocrosslinking strategy employing the non-canonical amino acid p-azidophenylalanine to study dimerization and cofactor interactions of the estrogen receptor α.
Item Type: | Thesis (Dissertation (Ph.D.)) | |||||||||
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Subject Keywords: | biochemistry; chemical biology; protein bioscience; electrophysiology; ion channels; neuroscience; signalling; molecular biolog | |||||||||
Degree Grantor: | California Institute of Technology | |||||||||
Division: | Chemistry and Chemical Engineering | |||||||||
Major Option: | Chemistry | |||||||||
Thesis Availability: | Public (worldwide access) | |||||||||
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Defense Date: | 31 May 2019 | |||||||||
Record Number: | CaltechTHESIS:06072019-131615199 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechTHESIS:06072019-131615199 | |||||||||
DOI: | 10.7907/X18Z-XE16 | |||||||||
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Default Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 11713 | |||||||||
Collection: | CaltechTHESIS | |||||||||
Deposited By: | Richard Mosesso | |||||||||
Deposited On: | 07 Jun 2019 22:16 | |||||||||
Last Modified: | 04 Oct 2019 00:26 |
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