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Phenotypic Antimicrobial Susceptibility Testing Based on Nucleic Acid Analysis


Schoepp, Nathan Garrett (2019) Phenotypic Antimicrobial Susceptibility Testing Based on Nucleic Acid Analysis. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/5F6B-F452.


Antimicrobial resistance (AMR) is one of the most widely recognized threats to global health, and one that continues to grow as new mechanisms of resistance evolve and resistant pathogens spread. Antibiotics are a cornerstone of modern medicine, but their misuse and overuse has constantly and consistently reduced their efficacy to the critically low levels we observe today. As a result, the rate of mortality as a direct result of AMR is approaching over a million deaths annually, with 20-year projections in the ten-millions. Rapid, phenotypic antimicrobial susceptibility testing (AST) that could be performed at the point of care (most notably in ≤ 30 min) would decrease the overuse of antimicrobials, allow physicians to make informed choices about which antimicrobials to prescribe, and improve patient outcomes. Today no such method exists. The ultimate goal of the below work is to allow physicians to choose, instead of guess, which antibiotics to use. We envision that development of these tests into distributable diagnostics will drastically improve patient outcomes, curb the spread of resistance, strengthen global antibiotic stewardship, and forestall the post-antibiotic era.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:AST, Nucleic Acids, DNA, Microfluidics, Antibiotics
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Ismagilov, Rustem
Thesis Committee:
  • Heath, James R. (chair)
  • Tirrell, David A.
  • Leadbetter, Jared R.
  • Ismagilov, Rustem F.
Defense Date:10 May 2019
Non-Caltech Author Email:nschoepp (AT)
Record Number:CaltechTHESIS:05302019-162535966
Persistent URL:
Related URLs:
URLURL TypeDescription adapted for Chapter II. adapted for Chapter III. adapted for Chapter IV. adapted for Chapter VII. adapted for Chapter VIII. adapted for Chapter IX.
Schoepp, Nathan Garrett0000-0002-2406-3693
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11572
Deposited By: Nathan Schoepp
Deposited On:31 May 2019 20:42
Last Modified:26 May 2021 01:56

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