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Phenotypic Antimicrobial Susceptibility Testing Based on Nucleic Acid Analysis

Citation

Schoepp, Nathan Garrett (2019) Phenotypic Antimicrobial Susceptibility Testing Based on Nucleic Acid Analysis. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/5F6B-F452. https://resolver.caltech.edu/CaltechTHESIS:05302019-162535966

Abstract

Antimicrobial resistance (AMR) is one of the most widely recognized threats to global health, and one that continues to grow as new mechanisms of resistance evolve and resistant pathogens spread. Antibiotics are a cornerstone of modern medicine, but their misuse and overuse has constantly and consistently reduced their efficacy to the critically low levels we observe today. As a result, the rate of mortality as a direct result of AMR is approaching over a million deaths annually, with 20-year projections in the ten-millions. Rapid, phenotypic antimicrobial susceptibility testing (AST) that could be performed at the point of care (most notably in ≤ 30 min) would decrease the overuse of antimicrobials, allow physicians to make informed choices about which antimicrobials to prescribe, and improve patient outcomes. Today no such method exists. The ultimate goal of the below work is to allow physicians to choose, instead of guess, which antibiotics to use. We envision that development of these tests into distributable diagnostics will drastically improve patient outcomes, curb the spread of resistance, strengthen global antibiotic stewardship, and forestall the post-antibiotic era.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:AST, Nucleic Acids, DNA, Microfluidics, Antibiotics
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Ismagilov, Rustem
Thesis Committee:
  • Heath, James R. (chair)
  • Tirrell, David A.
  • Leadbetter, Jared R.
  • Ismagilov, Rustem F.
Defense Date:10 May 2019
Non-Caltech Author Email:nschoepp (AT) pacbell.net
Record Number:CaltechTHESIS:05302019-162535966
Persistent URL:https://resolver.caltech.edu/CaltechTHESIS:05302019-162535966
DOI:10.7907/5F6B-F452
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/anie.201602763DOIArticle adapted for Chapter II.
http://dx.doi.org/10.1126/scitranslmed.aal3693DOIArticle adapted for Chapter III.
http://dx.doi.org/10.1038/s41598-018-29707-wDOIArticle adapted for Chapter IV.
http://dx.doi.org/10.1039/c6lc00292gDOIArticle adapted for Chapter VII.
http://dx.doi.org/10.1021/acs.analchem.6b01485DOIArticle adapted for Chapter VIII.
http://dx.doi.org/10.1021/acs.analchem.8b04324DOIArticle adapted for Chapter IX.
ORCID:
AuthorORCID
Schoepp, Nathan Garrett0000-0002-2406-3693
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11572
Collection:CaltechTHESIS
Deposited By: Nathan Schoepp
Deposited On:31 May 2019 20:42
Last Modified:22 Jun 2020 16:12

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