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Bioanalytical Tools to Develop Rapid Diagnostics and Study Physiology


Rolando, Justin Charles (2019) Bioanalytical Tools to Develop Rapid Diagnostics and Study Physiology. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/99NT-WP75.


This work details the development of bioanalytical tools for use in rapid diagnostics (Chapters 2-4) and in the study of physiology (Chapters 5-6). This research harness the power of real-time, singlemolecule microfluidics to study loop-mediated isothermal amplification in urinary tract infections (Chapter 2), chlamydia (Chapter 3), and gonorrhea (Chapter 4). In Chapter 5, non-reactive beads are designed and optimized to study the impact of polymers on murine gastrointestinal mucosa. Chapter 6 details the implementation of a mass spectrometry method to quantify bile acids and investigate their interaction with the microbiota in the murine gastro-intestinal tract.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Bioanalytical, Diagnostics, Physiology, loop-mediated isothermal amplification, urinary tract infections, chlamydia, gonorrhea, murine gastrointestinal mucosa, bile acids
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Ismagilov, Rustem
Thesis Committee:
  • Tirrell, David A. (chair)
  • Kornfield, Julia A.
  • Grubbs, Robert H.
  • Heath, James R.
  • Ismagilov, Rustem F.
Defense Date:10 May 2019
Funding AgencyGrant Number
Defense Threat Reduction Agency (DTRA)MCDC-18-01-01-00
Burroughs Wellcome FundInnovation in Regulatory Science Award
Defense Advanced Research Projects Agency (DARPA)HR0011-15-C-009
Defense Advanced Research Projects Agency (DARPA)HROO 11-17-2-0037
Defense Advanced Research Projects Agency (DARPA)HR0011-11-2-0006
Defense Advanced Research Projects Agency (DARPA)HROO1 1-17-2-0064
Joseph J. Jacobs Institute for Molecular Engineering for MedicineUNSPECIFIED
Record Number:CaltechTHESIS:05292019-112017023
Persistent URL:
Related URLs:
URLURL TypeDescription adapted for Ch. 2 adapted for Ch. 5
Rolando, Justin Charles0000-0001-8948-319X
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11559
Deposited By: Justin Rolando
Deposited On:01 Jun 2019 00:48
Last Modified:10 Feb 2020 16:57

Thesis Files

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