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The Control of RNA Synthesis in Isolated HeLa Cell Mitochondria

Citation

Gaines, George Loweree, III (1986) The Control of RNA Synthesis in Isolated HeLa Cell Mitochondria. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/1hj5-em11. https://resolver.caltech.edu/CaltechTHESIS:04172019-094731539

Abstract

The transcription of DNA and processing of RNA in mitochondria was investigated using isolated HeLa cell mitochondria. The intact organelles transcribe their DNA and process their RNA both qualitatively and quantitatively similar to the in vivo situation. Changing the conditions for the transcription reactions allowed the identification of a processing pathway for the small ribosomal RNA species, and an RNA species from the region surrounding the origin of light-strand replication with novel electrophoretic properties. Removal of the mitochondria from the cellular environment simplified the investigations of the nuclear-cytoplasmic influences upon the mitochondrial transcription. Differential sensitivities of all three transcription events, synthesizing the rRNAs, mRNAs, and light-strand RNAs, was shown to exist to a small molecular weight factor(s) present in the cytoplasm, and the availability of energetic substrates. These sensitivities indicate a link between cytoplasmic and respiratory/oxidative phosphorylative control of mitochondrial transcription.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Biology
Degree Grantor:California Institute of Technology
Division:Biology
Major Option:Biology
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Attardi, Giuseppe
Thesis Committee:
  • Attardi, Giuseppe (chair)
  • Emr, Scott D.
  • Abelson, John N.
  • Parker, Carl Stevens
  • Wold, Barbara J.
Defense Date:January 1986
Funders:
Funding AgencyGrant Number
Jean Weigle Memorial FundUNSPECIFIED
Record Number:CaltechTHESIS:04172019-094731539
Persistent URL:https://resolver.caltech.edu/CaltechTHESIS:04172019-094731539
DOI:10.7907/1hj5-em11
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/s0022-2836(84)80017-0DOIArticle adapted for Chapter 1.
https://doi.org/10.1128/mcb.4.8.1605DOIArticle adapted for Chapter 2.
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11478
Collection:CaltechTHESIS
Deposited By: Melissa Ray
Deposited On:17 Apr 2019 17:28
Last Modified:16 Apr 2021 23:12

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