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Investigation of the Catalytic Mechanism of Lysozyme using Substrate Analogs


Beranek, William, Jr. (1973) Investigation of the Catalytic Mechanism of Lysozyme using Substrate Analogs. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/H0X0-M137.


The catalytic mechanism of hen egg-white lysozyme is investigated by comparing the rate of hydrolysis of the trifluoro-Nacetyl analog of chitohexose to that of chitohexose. Since the binding properties of the two hexasaccharides are shown to be similar, differences observed in the rates of hydrolysis of the two oligosaccharides are discussed in terms of recent model studies for and mechanistic studies of lysozyme hydrolysis.

The role of distortion in the catalytic mechanism is investigated by comparing the kinetic parameters of the lysozyme-catalyzed hydrolysis of a xylose substrate analog with that of a glucose substrate analog.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Chemistry
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Raftery, Michael A.
Thesis Committee:
  • Unknown, Unknown
Defense Date:16 January 1973
Record Number:CaltechTHESIS:06292018-110142577
Persistent URL:
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11095
Deposited On:02 Jul 2018 16:47
Last Modified:21 Dec 2019 02:48

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