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Monoacetylation of 2,4-Diamino-6-Nitrotoluene Kinetic Studies with α-Chymotrypsin Effect of Dilantin Upon Ether Narcosis in the Cat

Citation

Foster, Robert Joseph (1952) Monoacetylation of 2,4-Diamino-6-Nitrotoluene Kinetic Studies with α-Chymotrypsin Effect of Dilantin Upon Ether Narcosis in the Cat. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/K13Z-9D57. https://resolver.caltech.edu/CaltechTHESIS:10052017-131007685

Abstract

The structure of the monoacetyl derivative of 2,4-diamino-6-nitrotoluene formed by reaction with an equimolar quantity of acetic anhydride in dry acetone at room temperature has been shown to be 2-amino-4-acetamido-6-nitrotoluene.

The kinetic constants for the enantiomorphic pair of acetyl-phecylalaninamides and α-chymotrypsin at 25°and pH 7.9 in aqueous solution have been determined. The relationships of these constants with others determined for similar enantiomorphic pairs is discussed. Equations for the competitive hydrol­ysis of two substrates at the same catalytically active site of an enzyme have been derived and have been tested experimentally.

The kinetic constants for L-tyrosine-hydroxamide and α-chymotrypsin have been determined at pH 6.9 and 25°. With this hydroxamide as substrate, the enzyme-inhibitor complex dis­sociation constants have been determined for a series of inhibitors at pH 6.9 and 25°. The differences between these constants and those at pH 7.9 is discussed and the charge state of the catalytically active site is inferred.

The effect of the anti-convulsant dilantin upon ether narcosis in the cat has been determined and is discussed in its relation to present theories of nervous propagation and narcosis.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Chemistry
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Niemann, Carl G.
Thesis Committee:
  • Unknown, Unknown
Defense Date:1 January 1952
Funders:
Funding AgencyGrant Number
CaltechUNSPECIFIED
Veterans AdministrationUNSPECIFIED
Record Number:CaltechTHESIS:10052017-131007685
Persistent URL:https://resolver.caltech.edu/CaltechTHESIS:10052017-131007685
DOI:10.7907/K13Z-9D57
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10488
Collection:CaltechTHESIS
Deposited By: Ben Perez
Deposited On:05 Oct 2017 20:44
Last Modified:21 Dec 2019 04:47

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