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Studies on the Purification and Properties of L-Leucine. Studies on the Mode of Action of Trypsin and Chymotrypsin

Citation

Thomas, Dudley Watson (1951) Studies on the Purification and Properties of L-Leucine. Studies on the Mode of Action of Trypsin and Chymotrypsin. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/DKCB-ER97. https://resolver.caltech.edu/CaltechTHESIS:09272017-150651665

Abstract

A procedure for the preparation of L-leucine is described and certain physical properties, useful for establishing the identity or purity, have been redetermined. The behavior of L-leucine in sulfuric acid and glacial acetic acid solutions has been investigated.

Preliminary investigations of the tryptic hydrolosis of acetyl- and benzoyl-L-argininamide have been conducted. It was found that aqueous trypsin solutions are quite unstable, and thus not ideally suited for kinetic studies. L-Arginine-methyl ester was found to be rapidly split at pH 4.0, this pH being far removed from the expected optimum (i.e. pH 7-8).

The kinetics of the α-chymotrypsin catalyzed hydrolysis of acetyl- and nicotinyl-L-tyrosinamide has been investigated at 25°C. and pH 7.8-8.0. Suitable rate expressions have been developed and the rate constants determined. The effect of various competitive inhibitors of α-chymotrypsin has been measured, and conclusions have been drawn relative to structure and affinity for the enzyme. It has been shown that the α-chymotryptic hydrolyses, so far investigated quantitatively, can be described in terms of the classical Michaelis-Menten enzyme-substrate complex theory.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Chemistry
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Niemann, Carl G.
Thesis Committee:
  • Unknown, Unknown
Defense Date:1 January 1951
Funders:
Funding AgencyGrant Number
CaltechUNSPECIFIED
Allied Chemical and Dye Corp.UNSPECIFIED
Record Number:CaltechTHESIS:09272017-150651665
Persistent URL:https://resolver.caltech.edu/CaltechTHESIS:09272017-150651665
DOI:10.7907/DKCB-ER97
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10464
Collection:CaltechTHESIS
Deposited By: Benjamin Perez
Deposited On:27 Sep 2017 23:12
Last Modified:21 Dec 2019 04:33

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