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The Course of Vitamin B₁ Metabolism in Man as Indicated by the Use of Radioactive Sulfur, a New Synthesis of 4-Methyl-5-β-Hudroxyethyl-Thiazole, and a Demonstration of the Use of Anti-Coincidence Method in Radioactivity Determinations

Citation

Hatcher, John Burton (1952) The Course of Vitamin B₁ Metabolism in Man as Indicated by the Use of Radioactive Sulfur, a New Synthesis of 4-Methyl-5-β-Hudroxyethyl-Thiazole, and a Demonstration of the Use of Anti-Coincidence Method in Radioactivity Determinations. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/7V9W-6E30. https://resolver.caltech.edu/CaltechTHESIS:08292017-161213519

Abstract

Thiamin synthesized from radiosulfur S35 was injected intramuscularly in two series of experiments, using a human subject on a normal and a B1-free diet. Determinations of the free B1 (by the thiochrome method) in the uring, the radiosulfur in the inorganic, ethereal, and neutral fractions of the urine, and the total radiosulfur in the feces were made. Rapid destruction of injected thiamin was indicated in both experiments by the appearance of radiosulfur in the inorganic fraction of the urine. Rapid interaction of the injected material with that pre-existing in the tissues was indicated by the appearance of free thiamin in the urine, without corresponding active sulfur. Additional destruction of thiamin was indicated by an excess of the neutral radiosulfur activity over the free B1 after the injections were stopped. After 36 days of the B1-free diet the injection of 8 mg. over a period of 3 days resulted in the excretion of 0.8 mg. of pre-existing thiamin. On the normal diet the injection of 63 mg. over a period of 4 days resulted in the excretion of 16 mg. of pre-existing thiamin.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:(Biochemistry and Chemistry)
Degree Grantor:California Institute of Technology
Division:Biology
Major Option:Biochemistry
Minor Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Unknown, Unknown
Thesis Committee:
  • Unknown, Unknown
Defense Date:1 January 1952
Record Number:CaltechTHESIS:08292017-161213519
Persistent URL:https://resolver.caltech.edu/CaltechTHESIS:08292017-161213519
DOI:10.7907/7V9W-6E30
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10398
Collection:CaltechTHESIS
Deposited By: Benjamin Perez
Deposited On:30 Aug 2017 14:38
Last Modified:11 May 2023 20:30

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