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I. The alpha-chymotrypsin-catalyzed and base-catalyzed hydrolysis of some substituted glycine esters and related compounds. II. The nature and properties of the enzyme-substrate complex produced under conditions of very high substrate concentrations. III. A hypothesis concerning alpha-chymotrypsin-substrate complexes

Citation

Wolf, John P. (1959) I. The alpha-chymotrypsin-catalyzed and base-catalyzed hydrolysis of some substituted glycine esters and related compounds. II. The nature and properties of the enzyme-substrate complex produced under conditions of very high substrate concentrations. III. A hypothesis concerning alpha-chymotrypsin-substrate complexes. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-02212006-134559

Abstract

A series of substituted glycogen esters have been evaluated as substrates for [alpha]-chymotrypsin. The second order base-catalyzed hydrolysis constants for these compounds have also been determined.

Four substrates for [alpha]-chymotrypsin have been found to exhibit unusual kinetic behavior at very high concentrations in the presence of [alpha]-chymotrypsin. It has been shown that these data can be the result of two molecules of substrate combining with each enzyme active site.

A hypothesis concerning the enzyme-substrate complex is offered which helps to correlate the data given in this work.

Item Type:Thesis (Dissertation (Ph.D.))
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Niemann, Carl G. (advisor)
  • Metcalf, Robert Lee (advisor)
Thesis Committee:
  • Unknown, Unknown
Defense Date:1 January 1959
Record Number:CaltechETD:etd-02212006-134559
Persistent URL:http://resolver.caltech.edu/CaltechETD:etd-02212006-134559
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:694
Collection:CaltechTHESIS
Deposited By: Imported from ETD-db
Deposited On:01 Mar 2006
Last Modified:26 Dec 2012 02:31

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