Wolf, John P. (1959) I. The alpha-chymotrypsin-catalyzed and base-catalyzed hydrolysis of some substituted glycine esters and related compounds. II. The nature and properties of the enzyme-substrate complex produced under conditions of very high substrate concentrations. III. A hypothesis concerning alpha-chymotrypsin-substrate complexes. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-02212006-134559
A series of substituted glycogen esters have been evaluated as substrates for [alpha]-chymotrypsin. The second order base-catalyzed hydrolysis constants for these compounds have also been determined.
Four substrates for [alpha]-chymotrypsin have been found to exhibit unusual kinetic behavior at very high concentrations in the presence of [alpha]-chymotrypsin. It has been shown that these data can be the result of two molecules of substrate combining with each enzyme active site.
A hypothesis concerning the enzyme-substrate complex is offered which helps to correlate the data given in this work.
|Item Type:||Thesis (Dissertation (Ph.D.))|
|Degree Grantor:||California Institute of Technology|
|Division:||Chemistry and Chemical Engineering|
|Thesis Availability:||Public (worldwide access)|
|Defense Date:||1 January 1959|
|Default Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Imported from ETD-db|
|Deposited On:||01 Mar 2006|
|Last Modified:||26 Dec 2012 02:31|
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