CaltechTHESIS
  A Caltech Library Service

A quantitative examination of the buoyancy behavior of macromolecules of known molecular weight in a density gradient at equilibrium in the ultracentrifuge

Citation

Ifft, James Brown (1962) A quantitative examination of the buoyancy behavior of macromolecules of known molecular weight in a density gradient at equilibrium in the ultracentrifuge. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechTHESIS:08182011-091559077

Abstract

The quantitative aspects of the method of sedimentation equilibrium in a density gradient with a purified protein of known molecular weight have been examined. In order to quantitatively evaluate the results of density gradient experiments, the density distribution and the density gradient in the salt solution at equilibrium in the ultracentrifuge must be known. Calculations based on thermodynamic data were made to determine these quantities. The results of this work are given in Part I. Because of the large hydrostatic pressures encountered during centrifugation, it was necessary to evaluate the effects of pressure on the results. In Part II, an experimental investigation of the changes in banding position of deoxyribonucleic acid (DNAY and tobacco mosaic virus (TMV) with pressure was made. A useful quantity involving the compressibilities of the solution and of the solvated macromolecule was obtained. The results agree with the thermodynamic theory presented. An experimental investigation of bovine serum mercaptalbumin (BMA) in a variety of salt solutions was carried out. Part III presents the experimental techniques and the methods of calculation for such experiments. The net hydration of BMA was determined solely from the ultracentrifuge results. The extent of anion binding was independently determined to obtain the net hydration of the protein-salt complex and the density gradient required to calculate the solvated molecular weight.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Chemistry
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Vinograd, Jerome Rubin
Thesis Committee:
  • Unknown, Unknown
Defense Date:1 January 1962
Record Number:CaltechTHESIS:08182011-091559077
Persistent URL:http://resolver.caltech.edu/CaltechTHESIS:08182011-091559077
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6593
Collection:CaltechTHESIS
Deposited By: John Wade
Deposited On:18 Aug 2011 16:37
Last Modified:26 Dec 2012 04:38

Thesis Files

[img]
Preview
PDF - Final Version
See Usage Policy.

29Mb

Repository Staff Only: item control page