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Pheromones in free-living and parasitic nematodes

Citation

Choe, Andrea (2012) Pheromones in free-living and parasitic nematodes. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechTHESIS:07042011-125318322

Abstract

Nematodes are among the most diverse phyla of animals, occupying almost every ecological niche available. Their ubiquity has led to a number of problems for civilization, including the loss of crops and the spread of neglected tropical diseases. Because they are responsible for a broad range of agricultural and human diseases, many pheromone-mediated nematode behaviors have been described but very few pheromones have been identified.

We report, via high-performance liquid chromatography electrospray ionization mass spectrometry, the discovery that many free-living and parasitic nematodes secrete small-molecule pheromones called ascarosides. These pheromones, called ascarosides, were first found to play a role in sex attraction and induction into a stress-resistant diapausal life stage in the free-living organism, Caenorhabditis elegans. We have performed a double-blind purification of the female sex pheromone in the sour paste nematode Panagrellus redivivus and report that the female sex pheromone is composed of at least two ascarosides. We have also found that both free-living and parasitic nematodes respond to different concentrations of ascarosides through attraction or repulsion, demonstrating cross-species communication. These results suggest that ascarosides could be a universal nematode cue, similar to the role of N-Acyl homoserine lactones in bacteria quorum sensing.

Because ascarosides are nonvolatile, they can only mediate close-range communication. Nematodes have a well-characterized capacity for long-range chemoattraction to a range of volatile cues. However, no studies have been done towards characterizing natural volatile cues derived from nematodes. Here I describe the discovery of volatile cues are produced by male-female species in the genus Caenorhabditis, but are lacking in the hermaphroditic species C. elegans, C. briggsae, and C. sp11. These volatile cues attract males (and sometimes females) from other Caenorhabditis species, demonstrating a cross-species gonochoristic cue.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:Pheromones, nematodes, free-living, parasitic, quorum, mate-finding
Degree Grantor:California Institute of Technology
Division:Biology
Major Option:Biology
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Sternberg, Paul W.
Thesis Committee:
  • Wold, Barbara J. (chair)
  • Mazmanian, Sarkis K.
  • Leadbetter, Jared R.
  • Platzer, Edward G.
  • Sternberg, Paul W.
Defense Date:17 June 2011
Author Email:ac (AT) caltech.edu
Record Number:CaltechTHESIS:07042011-125318322
Persistent URL:http://resolver.caltech.edu/CaltechTHESIS:07042011-125318322
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6536
Collection:CaltechTHESIS
Deposited By: Andrea Choe
Deposited On:25 Aug 2011 18:44
Last Modified:27 Dec 2013 02:31

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