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Outer-sphere effects on the copper sites of Pseudomonas aeruginosa azurins

Citation

Lancaster, Kyle M. (2011) Outer-sphere effects on the copper sites of Pseudomonas aeruginosa azurins. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechTHESIS:09082010-131422449

Abstract

This thesis describes structural, spectroscopic, and reactivity studies of mutants of the cupredoxin azurin from Pseudomonas aeruginosa. Emphasis is given to the effects of outer-sphere coordination on spectroscopic observables and reduction potentials. Among the key findings are that the characteristics of the classical "type 1" copper site can be largely reproduced in the absence of canonically necessary cysteine thiolate ligation. Mutants exhibiting this behavior have been classified as "type zero" copper proteins.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:bioinorganic chemistry; copper proteins; electron transfer; x-ray absorption spectroscopy; protein crystallography; electron paramagnetic resonance; azurin; cupredoxins
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Gray, Harry B.
Thesis Committee:
  • Richards, John H. (chair)
  • Mayo, Stephen L.
  • Winkler, Jay Richmond
  • Agapie, Theodor
  • Gray, Harry B.
Defense Date:18 August 2010
Funders:
Funding AgencyGrant Number
NIHDK019038
NSFGRF
StanfordGCEP
NSFCHE-0802907
NSFCHE-0553150
Record Number:CaltechTHESIS:09082010-131422449
Persistent URL:http://resolver.caltech.edu/CaltechTHESIS:09082010-131422449
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6023
Collection:CaltechTHESIS
Deposited By: Kyle Lancaster
Deposited On:28 Feb 2012 18:00
Last Modified:26 Dec 2012 04:30

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