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The pyrimidine oligonucleotide analysis of mitochondrial DNA from HeLa cells

Citation

Tong, Emmy (1972) The pyrimidine oligonucleotide analysis of mitochondrial DNA from HeLa cells. Master's thesis, California Institute of Technology. http://resolver.caltech.edu/CaltechTHESIS:04282010-143422774

Abstract

A pyrimidine cluster analysis of the light strand of HeLa mitochondrial DNA has been undertaken. The major difficulties in this attempt to obtain the pyrimidine oligonucleotide distribution in mitochondrial DNA are the limited amount of material available and the multistep procedure which may incur losses of DNA material as well as complications leading to irreproducible results. To overcome the scarcity of material, the sensitivity of isotopic labelling is enhanced by using the enzyme 5'-OH-polynucleotide kinase to attach a radioactive phosphate to the end of each oligonucleotide. The fingerprinting procedure is adapted to the microgram level of DNA material by thin layer displacement chromatography. The complications involved by multiple enzyme reactions have been solved by varying one parameter at a time. As a result of eight experiments, the conditions for a successful pyrimidine oligonucleotide analysis have been outlined.

Item Type:Thesis (Master's thesis)
Subject Keywords:Chemistry
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Unknown, Unknown
Thesis Committee:
  • Unknown, Unknown
Defense Date:1 January 1972
Record Number:CaltechTHESIS:04282010-143422774
Persistent URL:http://resolver.caltech.edu/CaltechTHESIS:04282010-143422774
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:5749
Collection:CaltechTHESIS
Deposited By: Tony Diaz
Deposited On:30 Jun 2010 16:07
Last Modified:26 Dec 2012 03:24

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