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pH titration studies of the acid denaturation of calf thymus deoxyribosenucleic acid

Citation

Wallace, Frederic A. (1960) pH titration studies of the acid denaturation of calf thymus deoxyribosenucleic acid. Master's thesis, California Institute of Technology. http://resolver.caltech.edu/CaltechTHESIS:04212010-142614826

Abstract

The acid denaturation of calf thymus deoxyribosenucleic acid (DNA) has been studied both by PH and spectrophotometric titration in the temperature range 10 - 30°C and at ionic strengths 0.1 F and 0.5 F. The number of hydrogen ions bound by the DNA at any given pH increased with increasing temperature and decreased with increasing ionic strength. The number bound at any given pH was greater after denaturation. A model for acid denaturation is proposed. The site of protonation of cytosine is discussed.

Item Type:Thesis (Master's thesis)
Subject Keywords:Chemistry
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Davidson, Norman R.
Thesis Committee:
  • Unknown, Unknown
Defense Date:1 January 1960
Record Number:CaltechTHESIS:04212010-142614826
Persistent URL:http://resolver.caltech.edu/CaltechTHESIS:04212010-142614826
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:5730
Collection:CaltechTHESIS
Deposited By: Tony Diaz
Deposited On:21 Jun 2010 18:24
Last Modified:26 Dec 2012 03:24

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