Zuschke-Baldwin, Brenda (1986) Investigations of the effect of antigen binding upon tryptophan reactivity in immunoglobulin G. Master's thesis, California Institute of Technology. http://resolver.caltech.edu/CaltechTHESIS:04052010-153132274
A ^3H/^(14)C-labelled reagent that reacts specifically with tryptophan residues, was used to monitor the reactivity of tryptophans in the Fc and Fab portions of IgG upon binding DNP-gly, (DNP)_1-cytochrome c, and (DNP)_8-cytochrome c. Binding of monovalent antigen caused a decrease in the reactivity of tryptophan residues in the Fc by 18%; binding of multivalent antigen caused decreases in the reactivity of tryptophan residues in Fc by 21% and Fab by 12%. Experiments to elucidate the interaction between IgG and complement component C4 were attempted. Future studies include further characterization of (DNP)_1-cytochrome c and-the complement fixation capability of IgM-ABPC22 with monovalent antigen. Also planned is an investigation of the interaction of C4 with IgM in soluble antibody-antigen complexes.
|Item Type:||Thesis (Master's thesis)|
|Degree Grantor:||California Institute of Technology|
|Division:||Chemistry and Chemical Engineering|
|Thesis Availability:||Restricted to Caltech community only|
|Defense Date:||June 1985|
|Default Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||19 Apr 2010 17:20|
|Last Modified:||26 Dec 2012 03:24|
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