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Investigations of the effect of antigen binding upon tryptophan reactivity in immunoglobulin G

Citation

Zuschke-Baldwin, Brenda (1986) Investigations of the effect of antigen binding upon tryptophan reactivity in immunoglobulin G. Master's thesis, California Institute of Technology. http://resolver.caltech.edu/CaltechTHESIS:04052010-153132274

Abstract

A ^3H/^(14)C-labelled reagent that reacts specifically with tryptophan residues, was used to monitor the reactivity of tryptophans in the Fc and Fab portions of IgG upon binding DNP-gly, (DNP)_1-cytochrome c, and (DNP)_8-cytochrome c. Binding of monovalent antigen caused a decrease in the reactivity of tryptophan residues in the Fc by 18%; binding of multivalent antigen caused decreases in the reactivity of tryptophan residues in Fc by 21% and Fab by 12%. Experiments to elucidate the interaction between IgG and complement component C4 were attempted. Future studies include further characterization of (DNP)_1-cytochrome c and-the complement fixation capability of IgM-ABPC22 with monovalent antigen. Also planned is an investigation of the interaction of C4 with IgM in soluble antibody-antigen complexes.

Item Type:Thesis (Master's thesis)
Subject Keywords:Chemistry
Degree Grantor:California Institute of Technology
Division:Chemistry and Chemical Engineering
Major Option:Chemistry
Thesis Availability:Restricted to Caltech community only
Research Advisor(s):
  • Unknown, Unknown
Thesis Committee:
  • Unknown, Unknown
Defense Date:June 1985
Record Number:CaltechTHESIS:04052010-153132274
Persistent URL:http://resolver.caltech.edu/CaltechTHESIS:04052010-153132274
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:5691
Collection:CaltechTHESIS
Deposited By: Tony Diaz
Deposited On:19 Apr 2010 17:20
Last Modified:26 Dec 2012 03:24

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