Quach, Quoc (Brandon) (2010) A parylene real time PCR microdevice. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechTHESIS:12142009-145646250
The polymerase chain reaction (PCR) is a powerful biochemical assay that is used in virtually all biochemical labs. By specifically amplifying a small sample of DNA, this technique is useful in the fields of paternity testing, forensics, and virus detection, just to name a few. A useful advancement of PCR involves monitoring the fluorescence generated by an increase in DNA during the amplification. This so called real time (RT)PCR allows quantification of the initial sample amount and allows for shorter assay times by stopping the reaction when enough fluorescence has been detected. Technology in the field of micro-electro-mechanical systems (MEMS) has advanced from the academic laboratory level to a handful of commercially successful devices. Work on adapting MEMS to biochemical applications, however, is still at the laboratory research stage. Recent breakthroughs in the use of more biocompatible materials in MEMS devices have helped to advance bio-MEMS. In particular, the polymer Parylene has superior properties that present a promising new platform for this field. This work presents the design, fabrication, and testing of a parylene-based MEMS RTPCR device. By combining advancements in both biology and MEMS engineering, this work demonstrates the feasibility of such a device along with quantitative analysis and data that serve as a guide for its future development.
|Item Type:||Thesis (Dissertation (Ph.D.))|
|Subject Keywords:||real time, PCR, MEMS, microfluidics, parylene|
|Degree Grantor:||California Institute of Technology|
|Division:||Engineering and Applied Science|
|Thesis Availability:||Public (worldwide access)|
|Defense Date:||4 December 2009|
|Author Email:||quoc (AT) mems.caltech.edu|
|Default Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Quoc Quach|
|Deposited On:||07 May 2010 21:48|
|Last Modified:||10 Dec 2014 20:26|
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