Electrical detection of DNA binding proteins

Citation

Gorodetsky, Alon A. (2009) Electrical detection of DNA binding proteins. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-12102008-101354

Abstract

The base pair stack of double helical DNA has proven to be an effective medium for charge transport. The π-stacked DNA base pairs can mediate charge transport (CT) chemistry over distances as long as 20 nm, and the reaction is exquisitely sensitive to DNA sequence-dependent conformation and dynamics. This sensitivity to perturbations in DNA structure and base pair stacking makes DNA-mediated charge transport chemistry an ideal methodology for the electrical detection of base mismatches, lesions, and protein binding. Efforts toward expanding the scope of electrochemistry at DNA-modified surfaces for biosensing applications are presented here.

Item Type:	Thesis (Dissertation (Ph.D.))
Subject Keywords:	DNA; electrochemistry; monolayers; proteins; self-assembly
Degree Grantor:	California Institute of Technology
Division:	Chemistry and Chemical Engineering
Major Option:	Chemistry
Thesis Availability:	Public (worldwide access)
Research Advisor(s):	Barton, Jacqueline K.
Thesis Committee:	Heath, James R. (chair) Gray, Harry B. Barton, Jacqueline K. Lewis, Nathan Saul
Defense Date:	1 December 2008
Record Number:	CaltechETD:etd-12102008-101354
Persistent URL:	http://resolver.caltech.edu/CaltechETD:etd-12102008-101354
Default Usage Policy:	No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:	5261
Collection:	CaltechTHESIS
Deposited By:	Imported from ETD-db
Deposited On:	03 Jun 2009
Last Modified:	26 Dec 2012 03:17