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Micronutrient requirements for Macrocystis pyrifera (L.) C.A. Agardh (giant kelp) gametophytes determined by means of a chemically defined medium, aquil

Citation

Kuwabara, James S. (1980) Micronutrient requirements for Macrocystis pyrifera (L.) C.A. Agardh (giant kelp) gametophytes determined by means of a chemically defined medium, aquil. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-12122006-091318

Abstract

Requirements of Macrocystis pyrifera (L.) C.A. Agardh gametophytes for nine nutrients (Co, Cu, Fe, I, Mn, Mo, N, P and Zn) were established by means of the chemically defined, artificial seawater, Aquil. Optimal growth ranges for total concentrations of Co, Cu, Fe, Mn, Zn and NO3-N and for media temperatures were determined. Effects on Macrocystis gametophytic growth within these optimal ranges were then investigated. Mathematical equations modeling gametophytic length as a function of concentrations of NO3-N, Fe and Zn, and Zn, Mn, Cu and Co were generated by least squares fit of experimental data. An Aquil formulation was developed that consistently produced Macrocystis embryonic sporophytes after 12 culturing days. Sporophyte production was achieved in Aquil using sporophylls from a variety of southern California sampling sites to initiate gametophytic cultures. This marks the first instance that any macroalga has been successfully cultured in a chemically defined artificial seawater medium using ultraclean techniques.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:ocean energy farming, giant kelp, micronutrients, trace metals, experimental design, statistical optimization
Degree Grantor:California Institute of Technology
Division:Engineering and Applied Science
Major Option:Environmental Science and Engineering
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • North, Wheeler J. (advisor)
  • Morgan, James J. (co-advisor)
Thesis Committee:
  • Unknown, Unknown
Defense Date:30 April 1980
Record Number:CaltechETD:etd-12122006-091318
Persistent URL:http://resolver.caltech.edu/CaltechETD:etd-12122006-091318
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4965
Collection:CaltechTHESIS
Deposited By: Imported from ETD-db
Deposited On:05 Jan 2007
Last Modified:26 Dec 2012 03:13

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