Kuwabara, James S. (1980) Micronutrient requirements for Macrocystis pyrifera (L.) C.A. Agardh (giant kelp) gametophytes determined by means of a chemically defined medium, aquil. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-12122006-091318
Requirements of Macrocystis pyrifera (L.) C.A. Agardh gametophytes for nine nutrients (Co, Cu, Fe, I, Mn, Mo, N, P and Zn) were established by means of the chemically defined, artificial seawater, Aquil. Optimal growth ranges for total concentrations of Co, Cu, Fe, Mn, Zn and NO3-N and for media temperatures were determined. Effects on Macrocystis gametophytic growth within these optimal ranges were then investigated. Mathematical equations modeling gametophytic length as a function of concentrations of NO3-N, Fe and Zn, and Zn, Mn, Cu and Co were generated by least squares fit of experimental data. An Aquil formulation was developed that consistently produced Macrocystis embryonic sporophytes after 12 culturing days. Sporophyte production was achieved in Aquil using sporophylls from a variety of southern California sampling sites to initiate gametophytic cultures. This marks the first instance that any macroalga has been successfully cultured in a chemically defined artificial seawater medium using ultraclean techniques.
|Item Type:||Thesis (Dissertation (Ph.D.))|
|Subject Keywords:||ocean energy farming, giant kelp, micronutrients, trace metals, experimental design, statistical optimization|
|Degree Grantor:||California Institute of Technology|
|Division:||Engineering and Applied Science|
|Major Option:||Environmental Science and Engineering|
|Thesis Availability:||Public (worldwide access)|
|Defense Date:||30 April 1980|
|Default Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Imported from ETD-db|
|Deposited On:||05 Jan 2007|
|Last Modified:||26 Dec 2012 03:13|
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