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Mechanosensitive Channels of Bacteria: Structure and Function. Electrophysiology as a High Resolution Technique of Ion Channel Study

Citation

Shapovalov, George G. (2005) Mechanosensitive Channels of Bacteria: Structure and Function. Electrophysiology as a High Resolution Technique of Ion Channel Study. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/XZ69-9A14. https://resolver.caltech.edu/CaltechETD:etd-12122004-215151

Abstract

Mechanosensitive (MS) ion channels commonly play a role of transducers converting mechanical stimuli into electrical or chemical signaling, thus allowing the cell to regulate its behavior in response to changing environment conditions. MS channels participate in sensation of sound and orientation in inner ear (hair cells), in touch sensation and in osmoregulation of bacteria. Structure of bacterial MS channels of large (MscL) and small (MscS) conductance has been recently solved at atomic resolution, stimulating various structural and functional studies. In this work author presents series of experiments enhancing an understanding of mechanosensation in bacteria. In Chapter 2 author performs cysteine cross-linking experiments suggesting asymmetric gating pattern of Tb-MscL ion channel. Chapters 3 and 4 establish a possibility of successful synthesis of fully functional Tb- and Ec-MscL proteins displaying a phenotype identical to recombinant channels. Studies in Chapter 5 and Appendix 1 extend the resolution of single-channel patch clamping technique, and describe a fine structure of MS channel gating by collecting and characterizing intersubstate transitions.

Item Type:Thesis (Dissertation (Ph.D.))
Subject Keywords:high resolution; ion channel; mechanosensitive; patch clamping; single channel; structure and function
Degree Grantor:California Institute of Technology
Division:Physics, Mathematics and Astronomy
Major Option:Physics
Thesis Availability:Public (worldwide access)
Research Advisor(s):
  • Lester, Henry A.
Thesis Committee:
  • Lester, Henry A. (chair)
  • Dougherty, Dennis A.
  • Rees, Douglas C.
  • Mabuchi, Hideo
  • Phillips, Robert B.
Defense Date:1 December 2004
Non-Caltech Author Email:george.shapovalov (AT) inserm.fr
Record Number:CaltechETD:etd-12122004-215151
Persistent URL:https://resolver.caltech.edu/CaltechETD:etd-12122004-215151
DOI:10.7907/XZ69-9A14
ORCID:
AuthorORCID
Shapovalov, George G.0000-0001-9702-9317
Default Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4962
Collection:CaltechTHESIS
Deposited By: Imported from ETD-db
Deposited On:07 Feb 2005
Last Modified:20 May 2020 21:46

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