Genetic and biochemical studies of the cystine-methionine series of mutants in Neurospora crassa

Citation

Fischer, Glenn Albert (1954) Genetic and biochemical studies of the cystine-methionine series of mutants in Neurospora crassa. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-12102003-111246

Abstract

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1. The following enzyme activities were studied in extracts of wild type and mutant Neurospora: [...]. Activity 2 was shown to be absent in a homocysteineless mutant and activity 3 was shown to be absent in a cystathionineless mutant. It was found that a suppressor, obtained by Giles, which causes these mutants to grow on minimal, returns enzyme activity to the mutants. Each activity is shown to be catalyzed by a different enzyme.

2. Evidence is presented which indicates that a Neurospora cystathionineless mutant can synthesize cystathionine from methionine without the intervention of cysteine.

3. Mutants which are blocked between thiosulfate and cysteine are shown to grow on elemental sulfur and H[subscript 2]S.

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